Hyperpolarized Binding Pocket Nuclear Overhauser Effect for Determination of Competitive Ligand Binding

被引：29

作者：

Lee, Youngbok ^{[1
]}

Zeng, Haifeng ^{[1
]}

Mazur, Adam ^{[2
]}

Wegstroth, Melanie ^{[2
]}

Carlomagno, Teresa ^{[3
]}

Reese, Marcel ^{[2
]}

Lee, Donghan ^{[2
]}

Becker, Stefan ^{[2
]}

Griesinger, Christian ^{[2
]}

Hilty, Christian ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem, Ctr Biol NMR, College Stn, TX 77843 USA

[2] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

[3] EMBL Heidelberg, D-69117 Heidelberg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 21期

基金：

美国国家科学基金会;

关键词：

hyperpolarization; ligands; NMR spectroscopy; proteins; POLARIZATION ENHANCED NMR; MAGNETIC-RESONANCE; DRUG DISCOVERY; PROTEIN; SPECTROSCOPY; RECEPTORS; XENON;

D O I：

10.1002/anie.201201003

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Protein-mediated polarization transfer: Ligands L1 and L2 that competitively bind to a protein are subject to indirect spin-polarization transfer through the binding site of the protein. If protons H L1 of one ligand are hyperpolarized by dynamic nuclear polarization (DNP, see picture), signal intensities in the NMR spectrum of the second ligand become enhanced. The relative build-up of signal of the second ligand yields information on its binding epitope. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：5179 / 5182

页数：4

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