Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro

被引：4

作者：

Hasegawa, Tomokazu ^{[1
]}

Chosa, Naoyuki ^{[2
]}

Asakawa, Takeyoshi ^{[1
]}

Yoshimura, Yoshitaka ^{[3
]}

Asakawa, Asami ^{[1
]}

Ishisaki, Akira ^{[2
]}

Tanaka, Mitsuro ^{[1
]}

机构：

[1] Iwate Med Univ, Sch Dent, Dept Pediat Dent, Morioka, Iwate 0208505, Japan

[2] Iwate Med Univ, Sch Dent, Dept Oral Biochem, Morioka, Iwate 0208505, Japan

[3] Hokkaido Univ, Div Oral Pathol Sci, Grad Sch Dent Med, Dept Mol Cell Pharmacol,Kita Ku, Sapporo, Hokkaido 0608586, Japan

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2010年 / 1卷 / 03期

关键词：

endothelial cell markers; dental pulp; deciduous teeth; PERIODONTAL-LIGAMENT CELLS; STEM-CELLS; ANGIOGENESIS;

D O I：

10.3892/etm_00000074

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Although dental pulp (DP) cells are indispensable for repair after injury, and blood supply is crucial for tissue healing and regeneration, the potential of DP cells derived from deciduous teeth to express endothelial cell (EC)-specific markers has yet to be determined. Therefore, this study investigated mRNA expression of the EC-specific markers vascular endothelial-cadherin (VE-cadherin), vascular endothelial growth factor receptor 2 (VEGFR2) and CD31 in DP cells derived from human deciduous teeth and treated with 10 ng/ml fibroblast growth factor (FGF)-2 in vitro. Quantitative PCR was used to determine the mRNA expression levels of the EC-specific markers, and showed that the FGF-2-cultured DP cells exhibited mRNA expression of VE-cadherin and VEGFR2, and marked CD31 mRNA expression. Western blot analysis showed that CD31 protein was induced in the DP cells following 3 weeks of treatment with FGF-2. DP cells derived from deciduous teeth inducibly expressed EC-specific markers, and thus have the potential to differentiate into cells of the vascular lineage.

引用

页码：477 / 480

页数：4

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