Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay

被引：7

作者：

Zar, Mian Sahib ^{[1
,2
,3
,4
,5
,6
]}

Shahid, Ahmad Ali ^{[5
]}

Shahzad, Muhammad Saqib ^{[5
]}

Shin, Kyoung-Jin ^{[6
]}

Lee, Hwan Young ^{[6
]}

Lee, Sang-Seob ^{[7
]}

Israr, Muhammad ^{[8
]}

Wiegand, Peter ^{[9
]}

Kulstein, Galina ^{[9
]}

机构：

[1] Chinese Acad Sci, Shenzhen Inst Adv Technol, Ctr Synthet Biol Engn Res CSynBER, 1068 Xuevuan Ave, Shenzhen, Peoples R China

[2] Chinese Acad Sci, Inst Biophys, Natl Lab, Beijing 100101, Peoples R China

[3] Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Beijing 100101, Peoples R China

[4] Univ Hlth Sci, Dept Forens Sci, Lahore, Pakistan

[5] Univ Punjab, CEMB, Lahore, Pakistan

[6] Yonsei Univ, Dept Forens Med, Coll Med, Seoul, South Korea

[7] Natl Forens Serv, Sect Forens Odontol, Med Examiners Off, 10 Ipchun Ro, Wonju, Gangwon Do, South Korea

[8] Univ Swat, Dept Forens Studies, Swat, Khyber Pakhtunk, Pakistan

[9] Ulm Univ, Inst Legal Med, Med Ctr, Albert Einstein Allee 23, D-89081 Ulm, Germany

来源：

JOURNAL OF FORENSIC SCIENCES | 2018年 / 63卷 / 06期

关键词：

forensic science; forensic genetics; forensic genotyping; skeletal remains; multiplex development; single-base extension; population genetics; single nucleotide polymorphism; IMPROVED DNA EXTRACTION; TOTAL DEMINERALIZATION; HUMAN PIGMENTATION; SKELETAL REMAINS; TECHNICAL NOTE; DEGRADED DNA; POLYMORPHISMS; AMPLIFICATION; SAMPLES; HERC2;

D O I：

10.1111/1556-4029.13783

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

This study introduces a newly developed in-house SNaPshot single-base extension (SBE) multiplex assay for forensic single nucleotide polymorphism (SNP) genotyping of fresh and degraded samples. The assay was validated with fresh blood samples from four different populations. In addition, altogether 24 samples from skeletal remains were analyzed with the multiplex. Full SNP profiles could be obtained from 14 specimens, while ten remains showed partial SNP profiles. Minor allele frequencies (MAF) of bone samples and different populations were compared and used for association of skeletal remains with a certain population. The results reveal that the SNPs of the bone samples are genetically close to the Pathan population. The findings show that the new multiplex system can be utilized for SNP genotyping of degraded and forensic relevant skeletal material, enabling to provide additional investigative leads in criminal cases.

引用

页码：1824 / 1829

页数：6

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