Cell penetrating peptide-mediated systemic siRNA delivery to the liver

被引：47

作者：

Hayashi, Yasuhiro ^{[1
]}

Yamauchi, Jun ^{[1
]}

Khalil, Ikramy A. ^{[1
]}

Kajimoto, Kazuaki ^{[1
]}

Akita, Hidetaka ^{[1
]}

Harashima, Hideyoshi ^{[1
]}

机构：

[1] Hokkaido Univ, Fac Pharmaceut Sci, Lab Mol Design Pharmaceut, Grad Sch Pharmaceut Sci,Kita Ku, Sapporo, Hokkaido 0600812, Japan

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2011年 / 419卷 / 1-2期

关键词：

Cell penetrating peptide; Octaarginine; siRNA delivery; Liver; SMALL INTERFERING RNA; IN-VIVO DELIVERY; GENE-EXPRESSION; OCTAARGININE; NANOPARTICLES; LIPOSOMES; STRATEGY; LIPIDS;

D O I：

10.1016/j.ijpharm.2011.07.038

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The cell-penetrating peptide (CPP) is one of the most attractive tools for efficiently delivering biomolecules to a target organelle. Here, we describe the use of octaarginine (R8)-modified lipid nanoparticles for the efficient and targeted in vivo delivery of siRNA to the liver. In this study, SR-BI (a scavenger receptor class B, member 1) was targeted by this nanoparticle. Our results demonstrate that R8-modified lipid nanoparticles can be used for the efficient and targeted delivery of liver siRNA to induce the specific knock-down of an endogenous gene with minimum liver toxicity and immune response, and that this CPP based technology holds considerable promise for further in vivo biological applications of siRNA. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：308 / 313

页数：6

共 27 条

[1] Nanoparticles for ex vivo siRNA delivery to dendritic cells for cancer vaccines: Programmed endosomal escape and dissociation [J].

Akita, Hidetaka ;

Kogure, Kentaro ;

Moriguchi, Rumiko ;

Nakamura, Yoshio ;

Higashi, Tomoko ;

Nakamura, Takashi ;

Serada, Satoshi ;

Fujimoto, Minoru ;

Naka, Tetsuji ;

Futaki, Shiroh ;

Harashima, Hideyoshi .

JOURNAL OF CONTROLLED RELEASE, 2010, 143 (03) :311-317

[2] RNA interference in mammalian cells by chemically-modified RNA [J].

Braasch, DA ;

Jensen, S ;

Liu, YH ;

Kaur, K ;

Arar, K ;

White, MA ;

Corey, DR .

BIOCHEMISTRY, 2003, 42 (26) :7967-7975

[3] Targeting cyclin B1 through peptide-based delivery of siRNA prevents tumour growth [J].

Crombez, Laurence ;

Morris, May Catherine ;

Dufort, Sandrine ;

Aldrian-Herrada, Gudrun ;

Nguyen, Quan ;

Mc Master, Gary ;

Coll, Jean-Luc ;

Heitz, Frederic ;

Divita, Gilles .

NUCLEIC ACIDS RESEARCH, 2009, 37 (14) :4559-4569

[4]

DEROSSI D, 1994, J BIOL CHEM, V269, P10444

[5] Cell-penetrating peptides: tools for intracellular delivery of therapeutics [J].

Deshayes, S ;

Morris, MC ;

Divita, G ;

Heitz, F .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2005, 62 (16) :1839-1849

[6] Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein [J].

Eguchi, Akiko ;

Meade, Bryan R. ;

Chang, Yung-Chi ;

Fredrickson, Craig T. ;

Willert, Karl ;

Puri, Nitin ;

Dowdy, Steven F. .

NATURE BIOTECHNOLOGY, 2009, 27 (06) :567-U110

[7] Cell-penetrating peptides:: Mechanisms and applications [J].

El-Andaloussi, S ;

Holm, T ;

Langel, Ü .

CURRENT PHARMACEUTICAL DESIGN, 2005, 11 (28) :3597-3611

[8] Octaarginine- and octalysine-modified nanoparticles have different modes of endosomal escape [J].

El-Sayed, Ayman ;

Khalil, Ikramy A. ;

Kogure, Kentaro ;

Futaki, Shiroh ;

Harashima, Hideyoshi .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (34) :23450-23461

[9] A DNA Microarray-based Analysis of the Host Response to a Nonviral Gene Carrier: A Strategy for Improving the Immune Response [J].

Hatakeyama, Hiroto ;

Ito, Erika ;

Yamamoto, Momoko ;

Akita, Hidetaka ;

Hayashi, Yasuhiro ;

Kajimoto, Kazuaki ;

Kaji, Noritada ;

Baba, Yoshinobu ;

Harashima, Hideyoshi .

MOLECULAR THERAPY, 2011, 19 (08) :1487-1498

[10] Specific gene expression of ATP-binding cassette transporters and nuclear hormone receptors in rat liver parenchymal, endothelial, and Kupffer cells [J].

Hoekstra, M ;

Kruijt, J ;

Van Eck, M ;

van Berkel, TJC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (28) :25448-25453

← 1 2 3 →