Molecular characterization of Brazilian infectious bursal disease viruses

被引:44
作者
Ikuta, N
El-Attrache, J
Villegas, P [1 ]
García, M
Lunge, VR
Fonseca, ASK
Oliveira, C
Marques, EK
机构
[1] Univ Georgia, Coll Vet Med, Dept Avian Med, Athens, GA 30602 USA
[2] Simbios Biotecnol, BR-90001970 Porto Alegre, RS, Brazil
[3] Univ Luterana Brasil, ULBRA, Canoas, RS, Brazil
[4] Merial Saude Anim Ltd, BR-13140000 Sao Paulo, Brazil
关键词
infectious bursal disease virus; IBDV; RT-PCR; Gumboro; field strains;
D O I
10.2307/1592968
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
A reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was used to amplify a VP2 gene fragment (248 bp) from infectious bursa disease virus (IBDV). The procedure allowed the detection of known IBDV strains from the United States, along with field isolates and commercial vaccines produced in Brazil. Amplified VP2 fragments were further characterized by restriction fragment length polymorphism (RFLP) analysis. From 55 Brazilian commercial flocks, 48 field samples were IBDV positive by RT-TCR. Vaccine RFLP patterns were found in 12 flocks, a pattern compatible with classic IBDV in one flock, four new patterns in 31 flocks, and a pattern compatible with very virulent (vv) IBDV in four flocks. Sequence analysis showed that the wIBDV RFLP patterns were closely related to the vvIBDVs described in Europe and Asia. Phylogenetic analysis of the four new RFLP patterns showed that they were closely related ro but distinct from other classic, variant, and wIBDVs, suggesting a high prevalence of different IBDV strains in Brazilian commercia flocks.
引用
收藏
页码:297 / 306
页数:10
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