Proteome analysis of abundant proteins in early Atlantic cod (Gadus morhua) larvae

被引:0
|
作者
Sveinsdottir, Holmfridur [1 ]
Gudmundsdottir, Agusta [2 ,3 ]
机构
[1] Matis Ohf, Iceland Food Res & Innovat, Div Biotechnol & Biomol, IS-550 Saudarkrokur, Iceland
[2] Univ Iceland, Sch Hlth Sci, Fac Food Sci & Nutr, IS-101 Reykjavik, Iceland
[3] Univ Iceland, Inst Sci, IS-107 Reykjavik, Iceland
来源
ICELANDIC AGRICULTURAL SCIENCES | 2011年 / 24卷
关键词
Atlantic cod larvae; proteome; 2-DE; MALDI-TOF MS; LC-MS/MS; 2-DIMENSIONAL ELECTROPHORESIS; SKELETAL-MUSCLE; EXPRESSION; ZEBRAFISH; HYPOXIA; SERUM;
D O I
暂无
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The production of good quality larvae is a challenge in marine fish hatcheries. In this study, the proteome profile of Atlantic cod (Gadus morhua) was analyzed using 2-DE, MALDI TOF MS and LC-MS/MS in order to determine its protein composition. Out of 109 abundant spots analyzed with MS 77 proteins were identified. The identified proteins were classified into 5 groups: structural proteins (56%), cytosolic proteins (29%), mitochondrial proteins (7%), secreted and extracellular proteins (7%) and nuclear proteins (1%). This study is the first step in a future construction of an Atlantic cod protein database that will be a valuable resource for molecular analysis of marine fish larval development.
引用
收藏
页码:23 / 31
页数:9
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