Role of synectin in lymphatic development in zebrafish and frogs

被引：34

作者：

Hermans, Karlien ^{[1
]}

Claes, Filip ^{[1
]}

Vandevelde, Wouter ^{[1
]}

Zheng, Wei ^{[2
]}

Geudens, Ilse ^{[1
]}

Orsenigo, Fabrizio ^{[3
]}

De Smet, Frederik ^{[1
]}

Gjini, Evisa ^{[4
,5
]}

Anthonis, Kristof ^{[1
]}

Ren, Bin ^{[6
]}

Kerjaschki, Dontcho ^{[7
]}

Autiero, Monica ^{[1
]}

Ny, Annelii ^{[1
]}

Simons, Michael ^{[6
]}

Dewerchin, Mieke ^{[1
]}

Schulte-Merker, Stefan ^{[4
,5
]}

Dejana, Elisabetta ^{[3
,8
]}

Alitalo, Kari ^{[2
]}

Carmeliet, Peter ^{[1
]}

机构：

[1] Vlaams Iustituut Biotechnol VIB, Vesalius Res Ctr, Leuven, Belgium

[2] Univ Helsinki, Haartman Inst, Biomed Helsinki, Mol Canc Biol Program, Helsinki, Finland

[3] IFOM, FIRC, Inst Mol Oncol, Milan, Italy

[4] Hubrecht Inst KNAW, Utrecht, Netherlands

[5] UMC, Utrecht, Netherlands

[6] Yale Univ, Sch Med, Sect Cardiovasc Med, New Haven, CT USA

[7] Med Univ Vienna, Inst Clin Pathol, Vienna, Austria

[8] Univ Milan, Dept Biomol Sci & Biotechnol, Milan, Italy

来源：

BLOOD | 2010年 / 116卷 / 17期

关键词：

ENDOTHELIAL-GROWTH-FACTOR; INTERACTING PROTEIN; REDUNDANT ROLES; VEGF-C; LYMPHANGIOGENESIS; SOX18; PROX1; GENE; SPECIFICATION; VASCULATURE;

D O I：

10.1182/blood-2009-11-254557

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The molecular basis of lymphangiogenesis remains incompletely characterized. Here, we document a novel role for the PDZ domain-containing scaffold protein synectin in lymphangiogenesis using genetic studies in zebrafish and tadpoles. In zebrafish, the thoracic duct arises from parachordal lymphangioblast cells, which in turn derive from secondary lymphangiogenic sprouts from the posterior cardinal vein. Morpholino knockdown of synectin in zebrafish impaired formation of the thoracic duct, due to selective defects in lymphangiogenic but not angiogenic sprouting. Synectin genetically interacted with Vegfr3 and neuropilin-2a in regulating lymphangiogenesis. Silencing of synectin in tadpoles caused lymphatic defects due to an underdevelopment and impaired migration of Prox-1(+) lymphatic endothelial cells. Molecular analysis further revealed that synectin regulated Sox18-induced expression of Prox-1 and vascular endothelial growth factor C-induced migration of lymphatic endothelial cells in vitro. These findings reveal a novel role for synectin in lymphatic development. (Blood. 2010; 116(17): 3356-3366)

引用

页码：3356 / 3366

页数：11

共 50 条

[1] Acquisition of SOX transcription factor specificity through protein-protein interaction, modulation of Wnt signalling and post-translational modification [J].

Bernard, Pascal ;

Harley, Vincent R. .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2010, 42 (03) :400-410

[2]

BUSSMANN J, 2010, DEVELOPMENT IN PRESS

[3] Sox18 and Sox7 play redundant roles in vascular development [J].

Cermenati, Solei ;

Moleri, Silvia ;

Cimbro, Simona ;

Corti, Paola ;

Del Giacco, Luca ;

Amodeo, Roberta ;

Dejana, Elisabetta ;

Koopman, Peter ;

Cotelli, Franco ;

Beltrame, Monica .

BLOOD, 2008, 111 (05) :2657-2666

[4] Selective regulation of arterial branching morphogenesis by synectin [J].

Chittenden, Thomas W. ;

Claes, Filip ;

Lanahan, Anthony A. ;

Autiero, Monica ;

Palac, Robert T. ;

Tkachenko, Eugene V. ;

Elfenbein, Arye ;

de Almodovar, Carmen Ruiz ;

Dedkov, Eduard ;

Tomanek, Robert ;

Li, Weiming ;

Westmore, Michael ;

Singh, JaiPal ;

Horowitz, Arie ;

Mulligan-Kehoe, Mary Jo ;

Moodie, Karen L. ;

Zhuang, Zhen W. ;

Carmeliet, Peter ;

Simons, Michael .

DEVELOPMENTAL CELL, 2006, 10 (06) :783-795

[5]

Cooke JE, 2001, DEVELOPMENT, V128, P571

[6] Distinct genetic interactions between multiple Vegf receptors are required for development of different blood vessel types in zebrafish [J].

Covassin, LD ;

Villefranc, JA ;

Kacergis, MC ;

Weinstein, BM ;

Lawson, ND .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (17) :6554-6559

[7] Neuropilin-2 interacts with VEGFR-2 and VEGFR-3 and promotes human endothelial cell survival and migration [J].

Favier, Benoit ;

Alam, Antoine ;

Barron, Pauline ;

Bonnin, Jacques ;

Laboudie, Patricia ;

Fons, Pierre ;

Mandron, Marie ;

Herault, Jean-Pascal ;

Neufeld, Gera ;

Savi, Pierre ;

Herbert, Jean-Marc ;

Bono, Francoise .

BLOOD, 2006, 108 (04) :1243-1250

[8] Sox18 induces development of the lymphatic vasculature in mice [J].

Francois, Mathias ;

Caprini, Andrea ;

Hosking, Brett ;

Orsenigo, Fabrizio ;

Wilhelm, Dagmar ;

Browne, Catherine ;

Paavonen, Karri ;

Karnezis, Tara ;

Shayan, Ramin ;

Downes, Meredith ;

Davidson, Tara ;

Tutt, Desmond ;

Cheah, Kathryn S. E. ;

Stacker, Steven A. ;

Muscat, George E. O. ;

Achen, Marc G. ;

Dejana, Elisabetta ;

Koopman, Peter .

NATURE, 2008, 456 (7222) :643-669

[9] Role of Delta-like-4/Notch in the Formation and Wiring of the Lymphatic Network in Zebrafish [J].

Geudens, Ilse ;

Herpers, Robert ;

Hermans, Karlien ;

Segura, Inmaculada ;

de Almodovar, Carmen Ruiz ;

Bussmann, Jeroen ;

De Smet, Frederik ;

Vandevelde, Wouter ;

Hogan, Benjamin M. ;

Siekmann, Arndt ;

Claes, Filip ;

Moore, John C. ;

Pistocchi, Anna Silvia ;

Loges, Sonja ;

Mazzone, Massimiliano ;

Mariggi, Giovanni ;

Bruyere, Francoise ;

Cotelli, Franco ;

Kerjaschki, Dontscho ;

Noel, Agnes ;

Foidart, Jean-Michel ;

Gerhardt, Holger ;

Ny, Annelii ;

Langenberg, Tobias ;

Lawson, Nathan D. ;

Duckers, Henricus J. ;

Schulte-Merker, Stefan ;

Carmeliet, Peter ;

Dewerchin, Mieke .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2010, 30 (09) :1695-1702

[10] Redundant roles for Sox7 and Sox18 in arteriovenous specification in zebrafish [J].

Herpers, Robert ;

van de Kamp, Esther ;

Duckers, Henricus J. ;

Schulte-Merker, Stefan .

CIRCULATION RESEARCH, 2008, 102 (01) :12-15

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