Differences in effects on DNA gyrase activity between two glutamate racemases of Bacillus subtilis, the poly-γ-glutamate synthesis-linking Glr enzyme and the YrpC (MurI) isozyme

被引：31

作者：

Ashiuchi, M ^{[1
]}

Kuwana, E

Komatsu, K

Soda, K

Misono, H

机构：

[1] Kochi Univ, Dept Bioresources Sci, Nankoku, Kochi 7838502, Japan

[2] Kansai Univ, Dept Biotechnol, Suita, Osaka 5648680, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 223卷 / 02期

关键词：

DNA gyrase; glutamate racemase; peptidoglycan; poly-gamma-glutamate; Bacillus subtilis;

D O I：

10.1016/S0378-1097(03)00381-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacillus subtilis possesses two isogenes encoding glutamate racemases, the poly-gamma-glutamate synthesis-linking Glr enzyme and the YrpC isozyme, and produces abundant amounts of the Glr enzyme. The YrpC isozyme, but not the Glr enzyme, was found to influence the activity of DNA gyrase. as did the Murl-type glutamate racemase of Escherichia coli, which is involved in peptidoglycan synthesis during cell division. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：221 / 225

页数：5

共 21 条

[1] Glutamate racemase is an endogenous DNA gyrase inhibitor [J].

Ashiuchi, M ;

Kuwana, E ;

Yamamoto, T ;

Komatsu, K ;

Soda, K ;

Misono, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (42) :39070-39073

[2] Biochemistry and molecular genetics of poly-γ-glutamate synthesis [J].

Ashiuchi, M ;

Misono, H .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 59 (01) :9-14

[3] Physiological and biochemical characteristics of poly γ-glutamate synthetase complex of Bacillus subtilis [J].

Ashiuchi, M ;

Nawa, C ;

Kamei, T ;

Song, JJ ;

Hong, SP ;

Sung, MH ;

Soda, K ;

Yagi, T ;

Misono, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (20) :5321-5328

[4]

Ashiuchi M, 1998, J BIOCHEM-TOKYO, V123, P1156

[5] Characterization of yrpC gene product of Bacillus subtilis IFO 3336 as glutamate racemase isozyme [J].

Ashiuchi, M ;

Soda, K ;

Misono, H .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1999, 63 (05) :792-798

[6] AN ESCHERICHIA-COLI GENE IN SEARCH OF A FUNCTION - PHENOTYPIC EFFECTS OF THE GENE RECENTLY IDENTIFIED AS MURI [J].

BALIKO, G ;

VENETIANER, P .

JOURNAL OF BACTERIOLOGY, 1993, 175 (20) :6571-6577

[7] DNA topoisomerase i from Mycobacterium smegmatis -: An enzyme with distinct features [J].

Bhaduri, T ;

Bagui, TK ;

Sikder, D ;

Nagaraja, V .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (22) :13925-13932

[8] IDENTIFICATION OF THE ESCHERICHIA-COLI MURI GENE, WHICH IS REQUIRED FOR THE BIOSYNTHESIS OF D-GLUTAMIC ACID, A SPECIFIC COMPONENT OF BACTERIAL PEPTIDOGLYCAN [J].

DOUBLET, P ;

VANHEIJENOORT, J ;

MENGINLECREULX, D .

JOURNAL OF BACTERIOLOGY, 1992, 174 (18) :5772-5779

[9] Regulation of the glutamate racemase of Escherichia coli investigated by site-directed mutagenesis [J].

Doublet, P ;

vanHeijenoort, J ;

MenginLecreulx, D .

MICROBIAL DRUG RESISTANCE, 1996, 2 (01) :43-49

[10]

FERRO AM, 1984, J BIOL CHEM, V259, P547

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