i-bodies, Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4

被引：47

作者：

Griffiths, Katherine ^{[1
]}

Dolezal, Olan ^{[2
]}

Cao, Benjamin ^{[3
,4
]}

Nilsson, Susan K. ^{[3
,4
]}

See, Heng B. ^{[5
,6
]}

Pfleger, Kevin D. G. ^{[5
,6
,7
]}

Roche, Michael ^{[8
,9
]}

Gorry, Paul R. ^{[10
]}

Pow, Andrew ^{[1
,13
]}

Viduka, Katerina ^{[1
,14
]}

Lim, Kevin ^{[1
,15
]}

Lu, Bernadine G. C. ^{[1
,16
]}

Chang, Denison H. C. ^{[1
,17
]}

Murray-Rust, Thomas ^{[1
]}

Kvansakul, Marc ^{[11
]}

Perugini, Matthew A. ^{[11
]}

Dogovski, Con ^{[11
,18
]}

Doerflinger, Marcel ^{[1
,19
]}

Zhang, Yuan ^{[12
]}

Parisi, Kathy ^{[1
,11
]}

Casey, Joanne L. ^{[1
,19
]}

Nuttall, Stewart D. ^{[2
]}

Foley, Michael ^{[1
,11
]}

机构：

[1] AdAlta Pty Ltd, 15-2 Pk Dr, Bundoora, Vic 3083, Australia

[2] CSIRO Mfg, Biomed Mfg, 343 Royal Parade, Parkville, Vic 3052, Australia

[3] Monash Univ, Australian Regenerat Med Inst, Wellington Rd, Clayton, Vic 3800, Australia

[4] CSIRO Mfg, Biomed Mfg, Bayview Ave, Clayton, Vic 3168, Australia

[5] Harry Perkins Inst Med Res, Nedlands, WA 6009, Australia

[6] Univ Western Australia, Ctr Med Res, Crawley, WA 6009, Australia

[7] Dimerix Biosci Ltd, Nedlands, WA 6009, Australia

[8] Univ Melbourne, Doherty Inst Infect & Immun, 792 Elizabeth St, Melbourne, Vic 3000, Australia

[9] Burnet Inst, 85 Commercial Rd, Melbourne, Vic 3004, Australia

[10] RMIT Univ, Coll Sci Engn & Hlth, Sch Hlth & Biomed Sci, Melbourne, Vic 3001, Australia

[11] La Trobe Univ, La Trobe Inst Mol Sci, Dept Biochem & Genet, Melbourne, Vic 3086, Australia

[12] Univ Melbourne, St Vincents Hosp, Dept Med, Fitzroy, Vic 3065, Australia

[13] Compugen Ltd, 260 East Grand Ave, San Francisco, CA 94080 USA

[14] AgriBio Ctr, Dept Econ Dev Jobs Transport & Resources, 5 Ring Rd, Bundoora, Vic 3083, Australia

[15] Imunexus, 343 Royal Parade, Parkville, Vic 3052, Australia

[16] CSL Ltd, 45 Poplar Rd, Parkville, Vic 3052, Australia

[17] Virbac, Milperra, NSW 2214, Australia

[18] Univ Melbourne, Dept Biochem & Mol Biol, Mol Sci & Biotechnol Inst Bio21, Melbourne, Vic 3010, Australia

[19] Nexvet, 21-31 Wright St, Clayton, Vic 3168, Australia

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2016年 / 291卷 / 24期

关键词：

HUMAN-IMMUNODEFICIENCY-VIRUS; HEMATOPOIETIC STEM; MONOCLONAL-ANTIBODIES; RAPID MOBILIZATION; CRYSTAL-STRUCTURE; ANTIGEN RECEPTOR; PROGENITOR CELLS; AMD3100; HIV-1; FRAGMENTS;

D O I：

10.1074/jbc.M116.721050

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CXCR4 is a G protein-coupled receptor with excellent potential as a therapeutic target for a range of clinical conditions, including stem cell mobilization, cancer prognosis and treatment, fibrosis therapy, and HIV infection. We report here the development of a fully human single-domain antibody-like scaffold termed an "i-body," the engineering of which produces an i-body library possessing a long complementarity determining region binding loop, and the isolation and characterization of a panel of i-bodies with activity against human CXCR4. The CXCR4-specific i-bodies show antagonistic activity in a range of in vitro and in vivo assays, including inhibition of HIV infection, cell migration, and leukocyte recruitment but, importantly, not the mobilization of hematopoietic stem cells. Epitope mapping of the three CXCR4 i-bodies AM3-114, AM4-272, and AM3-523 revealed binding deep in the binding pocket of the receptor.

引用

页码：12641 / 12657

页数：17

共 50 条

[1] Imaging agents for the chemokine receptor 4 (CXCR4)
Kuil, Joeri
Buckle, Tessa
van Leeuwen, Fijs W. B.
CHEMICAL SOCIETY REVIEWS, 2012, 41 (15) : 5239 - 5261
[2] Solubilization of the chemokine receptor CXCR4
Staudinger, R
Bandres, JC
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 274 (01) : 153 - 156
[3] Soluble chemokine receptor CXCR4 is present in human sera
Malvoisin, Etienne
Livrozet, Jean-Michel
Makloufi, Djamila
Vincent, Nadine
ANALYTICAL BIOCHEMISTRY, 2011, 414 (02) : 202 - 207
[4] CXCR4 chemokine receptor antagonists: perspectives in SCLC
Burger, Jan A.
Stewart, David J.
EXPERT OPINION ON INVESTIGATIONAL DRUGS, 2009, 18 (04) : 481 - 490
[5] Inhibition of the human chemokine receptor CXCR4 by antisense phosphorothioate oligodeoxyribonucleotides
Kusunoki, A
Saitou, T
Miyano-Kurosaki, N
Takaku, H
FEBS LETTERS, 2001, 488 (1-2) : 64 - 68
[6] Antibodies Targeting Chemokine Receptors CXCR4 and ACKR3
Bobkov, Vladimir
Arimont, Marta
Zarca, Aurelien
De Groof, Timo W. M.
van der Woning, Bas
de Haard, Hans
Smit, Martine J.
MOLECULAR PHARMACOLOGY, 2019, 96 (06) : 753 - 764
[7] Crystal structure of the chemokine receptor CXCR4 in complex with a viral chemokine
Qin, Ling
Kufareva, Irina
Holden, Lauren G.
Wang, Chong
Zheng, Yi
Zhao, Chunxia
Fenalti, Gustavo
Wu, Huixian
Han, Gye Won
Cherezov, Vadim
Abagyan, Ruben
Stevens, Raymond C.
Handel, Tracy M.
SCIENCE, 2015, 347 (6226) : 1117 - 1122
[8] Kinetic Analysis of the Early Signaling Steps of the Human Chemokine Receptor CXCR4
Perpina-Viciano, Cristina
Isbilir, Ali
Zarca, Aurelien
Caspar, Birgit
Kilpatrick, Laura E.
Hill, Stephen J.
Smit, Martine J.
Lohse, Martin J.
Hoffmann, Carsten
MOLECULAR PHARMACOLOGY, 2020, 98 (02) : 72 - 87
[9] Structure-Based Development of Antagonists for Chemokine Receptor CXCR4
Zhang, Chongqian
Hou, Tingjun
Feng, Zhiwei
Li, Youyong
CURRENT COMPUTER-AIDED DRUG DESIGN, 2013, 9 (01) : 60 - 75
[10] CXC chemokine receptor 4 (CXCR4) blockade in cancer treatment
Bao, Shunshun
Darvishi, Mohammad
Amin, Ali
Al-Haideri, Maysoon T.
Patra, Indrajit
Kashikova, Khadisha
Ahmad, Irfan
Alsaikhan, Fahad
Al-qaim, Zahraa Haleem
Al-Gazally, Moaed E.
Kiasari, Bahman Abedi
Tavakoli-Far, Bahareh
Sidikov, Akmal A.
Mustafa, Yasser Fakri
Akhavan-Sigari, Reza
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2023, 149 (10) : 7945 - 7968

← 1 2 3 4 5 →