Electrical membrane properties and ionic currents in cultured goldfish gonadotrophs

被引:30
作者
VanGoor, F [1 ]
Goldberg, JI [1 ]
Chang, JP [1 ]
机构
[1] UNIV ALBERTA,DEPT BIOL SCI,EDMONTON,AB T6G 2E9,CANADA
关键词
calcium current; sodium current; potassium current;
D O I
10.1139/cjpp-74-6-729
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The electrical membrane properties and ionic currents of cultured identified goldfish gonadotrophs were characterized using whole-cell patch-clamp recordings. Under current-clamp recording conditions, goldfish gonadotrophs had a resting membrane potential of approximate to-60 mV and an input resistance of 8.2 +/- 1.6 G Ohm. In response to depolarizing current injections, one or more action potentials could be induced from resting membrane potential or more hyperpolarized holding potentials. In addition, some cells exhibited spontaneous action potential activity. Under voltage-clamp recording conditions, several outward and inward currents were isolated and characterized using pharmacological and ionic substitution studies. The outward currents included a fast-activating, transient current and an inactivation-resistant current similar to I-A-type and delayed rectifier currents, respectively. Unlike mammalian gonadotrophs, apamin-sensitive K+ currents were not detected. Inward currents included a fast-activating and inactivating Na+ current and a high-voltage activated, dihydropyridine-sensitive Ca2+ current. The value for half-maximal steady-state inactivation of the Na+ and Ca2+ currents was -50.8 and -16.7 mV, respectively, indicating that a significant proportion of both Na+ and Ca2+ channels are available for activation at the resting membrane potential of these cells.
引用
收藏
页码:729 / 743
页数:15
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