Autophagy is involved in anti-viral activity of pentagalloylglucose (PGG) against Herpes simplex virus type 1 infection in vitro

被引：39

作者：

Pei, Ying ^{[1
]}

Chen, Zhen-Ping ^{[1
]}

Ju, Huai-Qiang ^{[1
]}

Komatsu, Masaaki ^{[2
]}

Ji, Yu-hua ^{[3
]}

Liu, Ge ^{[4
]}

Guo, Chao-wan ^{[4
]}

Zhang, Ying-Jun ^{[5
]}

Yang, Chong-Ren ^{[5
]}

Wang, Yi-Fei ^{[1
]}

Kitazato, Kaio ^{[4
]}

机构：

[1] Jinan Univ, Biomed Res & Dev Ctr, Guangzhou 510632, Guangdong, Peoples R China

[2] Tokyo Metropolitan Inst Med Sci, Lab Frontier Sci, Bunkyo Ku, Tokyo 1138613, Japan

[3] Jinan Univ, Coll Life Sci & Technol, Inst Tissue Transplantat & Immunol, Guangzhou 510632, Guangdong, Peoples R China

[4] Nagasaki Univ, Grad Sch Biomed Sci, Dept Mol Microbiol & Immunol, Div Mol Pharmacol Infect Agents, Nagasaki 8528521, Japan

[5] Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 405卷 / 02期

基金：

美国国家科学基金会;

关键词：

Autophagy; Pentagalloylglucose (PGG); HSV-1; infections; Anti-viral activity; SIGNALING PATHWAY; MAMMALIAN TARGET; CELLS; PROTEIN; KINASE; 1,2,3,4,6-PENTA-O-GALLOYL-BETA-D-GLUCOSE; INHIBITION; EXPRESSION; COMPLEX; CANCER;

D O I：

10.1016/j.bbrc.2011.01.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pentagalloylglucose (PGG) is a natural polyphenolic compound with broad-spectrum anti-viral activity, however, the mechanisms underlying anti-viral activity remain undefined. In this study, we investigated the effects of PGG on anti-viral activity against Herpes simplex virus type 1 (HSV-1) associated with autophagy. We found that the PGG anti-HSV-1 activity was impaired significantly in MEF-atg7(-/-) cells (autophagy-defective cells) derived from an atg7(-/-) knockout mouse. Transmission electron microscopy revealed that PGG-induced autophagosomes engulfed HSV-1 virions. The mTOR signaling pathway, an essential pathway for the regulation of autophagy, was found to be suppressed following PGG treatment. Data presented in this report demonstrated for the first time that autophagy induced following PGG treatment contributed to its anti-HSV activity in vitro. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：186 / 191

页数：6

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