Effect of mosquito midgut trypsin activity on dengue-2 virus infection and dissemination in Aedes aegypti

被引：75

作者：

Molina-Cruz, A ^{[1
]}

Gupta, L

Richardson, J

Bennett, KF

Black, W

Barillas-Mury, C

机构：

[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

[2] Colorado State Univ, Anthropod Borne & Infect Dis Lab, Ft Collins, CO 80523 USA

[3] NIAID, Lab Malaria & Vector Res, Med Entomol Sect, NIH, Rockville, MD USA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2005年 / 72卷 / 05期

关键词：

D O I：

10.4269/ajtmh.2005.72.631

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The effect of mosquito midgut trypsins in dengue serotype 2 flavivirus (DENV-2) infectivity to Aedes aegypti was studied. Addition of soybean trypsin inhibitor (STI) in a DENV-2 infectious blood meal resulted in a 91-97% decrease in midgut DENV-2 RNA copies (qRT-PCR analysis). STI treatment also resulted in slower DENV-2 replica:ion in the midgut, less DENV-2 E protein expression, and decreased dissemination to the thorax and the head. A second uninfected blood meal, 7 days after the STI-treated infectious meal, significantly increased DENV-2 replication in the midgut and recovered oogenesis, suggesting that the lower viral infection caused by STI was in part due to a nutritional effect. Mosquitoes fed DENV-2 digested in vitro with bovine trypsin (before STI addition) exhibited a transient increase in midgut DENV-2 4 days postinfection. Blood digestion and possibly DENV-2 proteolytic processing, mediated by midgut trypsins, influence the rate of DENV-2 infection, replication, and dissemination in Ae. aegypti.

引用

页码：631 / 637

页数：7

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