Micropatterning proteins and cells on polylactic acid and poly(lactide-co-glycolide)

被引：56

作者：

Lin, CC ^{[1
]}

Co, CC ^{[1
]}

Ho, CC ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Chem & Mat Engn, Cincinnati, OH 45221 USA

来源：

BIOMATERIALS | 2005年 / 26卷 / 17期

关键词：

micropatterning; poly(lactide-co-glycolide) (PLGA); poly(lactide acid) (PLA); poly(ethylene glycol); tissue engineering;

D O I：

10.1016/j.biomaterials.2004.09.051

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Techniques for micropatterning proteins and cells on biomaterials are important in tissue engineering applications. Here, we present a method for patterning proteins and cells on poly(lactic acid) (PLA) and poly(lactide-co-glycolide) (PLGA) substrates that are routinely used as scaffolds in engineering tissues. Poly(oligoethyleneglycol methacrylate) (poly-OEGMA) or poly(oligoethyleneglycol methacrylate-co-methacrylic acid) (poly(OEGMA-co-MA)) was microcontact printed onto substrates to create cell resistant areas. Proteins adsorbed onto the imprinted regions whereas the polymer printed regions effectively repel non-specific protein adsorption. NIH 3T3 fibroblasts remain confined within the patterns on the PLGA and PLA films for up to 2 weeks and aligned their actin cytoskeleton along the line patterns. In comparison to unpatterned cells, fibroblasts confined within line-shaped patterns show fewer actin filaments. This method for controlling the spatial morphology and distribution of cells on synthetic biomaterials could have significant applications in tissue engineering. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：3655 / 3662

页数：8

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