Different osteogenic differentiation potential of mesenchymal stem cells on three different polymeric substrates

被引：34

作者：

Abazari, Mohammad Foad ^{[1
]}

Hosseini, Zahrasadat ^{[2
]}

Karizi, Shohreh Zare ^{[3
]}

Norouzi, Sara ^{[4
]}

Faskhoudi, Mojdeh Amini ^{[5
]}

Saburi, Ehsan ^{[6
]}

Enderami, Seyed Ehsan ^{[7
]}

Ardeshirylajimi, Abdolreza ^{[8
]}

Mohajerani, Hassan ^{[9
]}

机构：

[1] Univ Tehran Med Sci, Res Ctr Clin Virol, Tehran, Iran

[2] Kharazmi Univ, Dept Biol, Tehran, Iran

[3] Islamic Azad Univ, Dept Biol, Varamin Pishva Branch, Pishva, Varamin, Iran

[4] Islamic Azad Univ, Fac Sci & Res, Dept Biol, Tehran, Iran

[5] Islamic Azad Univ, Dept Biol, Tehran Med Branch, Tehran, Iran

[6] Mashhad Univ Med Sci, Med Genet & Mol Med Dept, Mashhad, Razavi Khorasan, Iran

[7] Massandaran Univ Med Sci, Immunogenet Res Ctr, Sch Adv Technol Med, Dept Med Biotechnol, Sari, Iran

[8] St Louis Univ, Edward A Doisy Dept Biochem & Mol Biol, St Louis, MO 63103 USA

[9] Shahid Beheshti Univ Med Sci, Dent Sch, Dept Oral & Maxillofacial Surg, Tehran, Iran

来源：

GENE | 2020年 / 740卷

关键词：

Osteogenesis; Nanofibrous substrate; Mesenchymal stem cells; Gene expression; IN-VIVO DEGRADATION; OF-THE-ART; NATURAL POLYMERS; TISSUE; SCAFFOLDS; BIOMATERIALS; PCL;

D O I：

10.1016/j.gene.2020.144534

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The function of tissue cells is strongly depends on the extracellular matrix (ECM) that can guide and support cell structure. This support plays a crucial role in the process of cell proliferation and differentiation. Herein, three different nanofibrous scaffolds that are highly attractive for tissue engineering were selected and then osteogenic related genes and protein expression patterns of human adipose-derived mesenchymal stem cells (AT-MSCs) were investigated when grown on substrates. Polycaprolactone, Poly (L-lactic acid) and Polyvinylidene-fluoride nanofibrous scaffolds were fabricated using Electrospinning method and then AT-MSCs viability and osteogenic differentiation were evaluated while cultured on them. The highest AT-MSCs survival rate when grown on the scaffolds was detected when grown on Polyvinylidene-fluoride. In addition, the highest ALP activity and mineralization were also observed in differentiated AT-MSCs has grown on Polyvinylidene-fluoride. The expression levels of Runx2, osteonectin and osteocalcin genes and osteocalcin protein in the AT-MSCs has grown on the Polyvinylidene-fluoride were also significantly higher than the rest of the scaffolds. Based on the results, it seems that since the studied substrate have a similar structural characteristics, their nature may have an important role in the stem cell's osteogenesis process, where the Polyvinylidene-fluoride piezoelectricity was a most distinguished characteristic.

引用

页数：7

共 37 条

[21] Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds [J].