miR-205-5p Mediated Downregulation of PTEN Contributes to Cisplatin Resistance in C13K Human Ovarian Cancer Cells

被引：39
作者：
Shi, Xiaoyan [1 ,3 ]
Xiao, Lan [4 ]
Mao, Xiaolu [5 ]
He, Jinrong [1 ,3 ]
Ding, Yu [1 ,3 ]
Huang, Jin [1 ,3 ]
Peng, Caixia [1 ,3 ]
Xu, Zihui [1 ,2 ]
机构：
[1] Huazhong Univ Sci & Technol, Cent Hosp Wuhan, Tongji Med Coll, Key Lab Mol Diag Hubei Prov, Wuhan, Hubei, Peoples R China
[2] Wuhan Univ, Renmin Hosp, Dept Endocnnol & Metab, Wuhan, Hubei, Peoples R China
[3] Huazhong Univ Sci & Technol, Cent Hosp Wuhan, Tongji Med Coll, Cent Lab, Wuhan, Hubei, Peoples R China
[4] An Hui Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Hefei, Anhui, Peoples R China
[5] Huazhong Univ Sci & Technol, Cent Hosp Wuhan, Tongii Med Coll, Dept Clin Lab, Wuhan, Hubei, Peoples R China
来源：
FRONTIERS IN GENETICS | 2018年 / 9卷
基金：
中国国家自然科学基金;
关键词：
miR-205-5p; PTEN; cisplatin resistance; ovarian cancer; AKT; PANCREATIC-CANCER; INDUCED APOPTOSIS; TARGETING PTEN; LUNG-CANCER; INHIBITION; EXPRESSION; CARCINOMA; PATHWAY; LEADS;
D O I：
10.3389/fgene.2018.00555
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Cisplatin resistance is a major cause of treatment failure in advanced ovarian cancer. The limited evidence shows the paradoxical regulation of miR-205 on chemotherapy resistance in cancer. Herein, we found that miR-205-5p was enormously increased in cisplatin-resistant C13K ovarian cancer cells compared with its cisplatin-sensitive OV2008 parental cells using miRNA microarrays, which was further verified by quantitative PCR. Furthermore, we confirmed that inhibition of miR-205-5p upregulated PTEN and subsequently attenuated its downstream target p-AKT, which inversed C13K cells from cisplatin resistance to sensitivity. Our data suggest that miR-205-5p contributes to cisplatin resistance in C13K ovarian cancer cells may via targeting PTEN/AKT pathway.
引用
收藏
页数：8
相关论文
共 30 条
[1]   E2F1 confers anticancer drug resistance by targeting ABC transporter family members and Bcl-2 via the p73/DNp73-miR-205 circuitry [J].
Alla, Vijay ;
Kowtharapu, Bhavani S. ;
Engelmann, David ;
Emmrich, Stephan ;
Schmitz, Ulf ;
Steder, Marc ;
Puetzer, Brigitte M. .
CELL CYCLE, 2012, 11 (16) :3067-3078
[2]  
Asselin E, 2001, CANCER RES, V61, P1862
[3]   A miRNA Signature of Chemoresistant Mesenchymal Phenotype Identifies Novel Molecular Targets Associated with Advanced Pancreatic Cancer [J].
Bera, Alakesh ;
VenkataSubbaRao, Kolaparthi ;
Manoharan, Muthu Saravanan ;
Hill, Ping ;
Freeman, James W. .
PLOS ONE, 2014, 9 (09)
[4]   Downregulation of miR-205 and miR-31 confers resistance to chemotherapy-induced apoptosis in prostate cancer cells [J].
Bhatnagar, N. ;
Li, X. ;
Padi, S. K. R. ;
Zhang, Q. ;
Tang, M-S ;
Guo, B. .
CELL DEATH & DISEASE, 2010, 1 :e105-e105
[5]   miR-205 Targets PTEN and PHLPP2 to Augment AKT Signaling and Drive Malignant Phenotypes in Non-Small Cell Lung Cancer [J].
Cai, Junchao ;
Fang, Lishan ;
Huang, Yongbo ;
Li, Rong ;
Yuan, Jie ;
Yang, Yi ;
Zhu, Xun ;
Chen, Baixue ;
Wu, Jueheng ;
Li, Mengfeng .
CANCER RESEARCH, 2013, 73 (17) :5402-5415
[6]   New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase AKT pathway [J].
Cantley, LC ;
Neel, BG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (08) :4240-4245
[7]   Chemosensitization and inhibition of pancreatic cancer stem cell proliferation by overexpression of microRNA-205 [J].
Chaudhary, Amit Kumar ;
Mondal, Goutam ;
Kumar, Virender ;
Kattel, Krishna ;
Mahato, Ram I. .
CANCER LETTERS, 2017, 402 :1-8
[8]   miR-205 regulates the proliferation and invasion of ovarian cancer cells via suppressing PTEN/SMAD4 expression [J].
Chu, Ping ;
Liang, Aihua ;
Jiang, Aili ;
Zong, Lu .
ONCOLOGY LETTERS, 2018, 15 (05) :7571-7578
[9]   miR-205-5p-mediated downregulation of ErbB/HER receptors in breast cancer stem cells results in targeted therapy resistance [J].
De Cola, A. ;
Volpe, S. ;
Budani, M. C. ;
Ferracin, M. ;
Lattanzio, R. ;
Turdo, A. ;
D'Agostino, D. ;
Capone, E. ;
Stassi, G. ;
Todaro, M. ;
Di Ilio, C. ;
Sala, G. ;
Piantelli, M. ;
Negrini, M. ;
Veronese, A. ;
De laurenzi, V. .
CELL DEATH & DISEASE, 2015, 6 :e1823-e1823
[10]   Systems biology of cisplatin resistance: past, present and future [J].
Galluzzi, L. ;
Vitale, I. ;
Michels, J. ;
Brenner, C. ;
Szabadkai, G. ;
Harel-Bellan, A. ;
Castedo, M. ;
Kroemer, G. .
CELL DEATH & DISEASE, 2014, 5 :e1257-e1257
123