High-Resolution 3-D Refractive Index Tomography and 2-D Synthetic Aperture Imaging of Live Phytoplankton

被引:28
|
作者
Lee, SangYun [1 ]
Kim, Kyoohyun [1 ]
Mubarok, Adam [2 ]
Panduwirawan, Adisetyo [2 ]
Lee, KyeoReh [1 ]
Lee, Shinhwa [3 ]
Park, HyunJoo [1 ]
Park, YongKeun [1 ]
机构
[1] Korea Adv Inst Sci & Technol, Dept Phys, Taejon 305701, South Korea
[2] Bandung Inst Technol, Dept Phys, Bandung, Indonesia
[3] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea
基金
新加坡国家研究基金会;
关键词
Phytoplankton; Plankton; Quantitative phase imaging; Diffraction optical tomography; OPTICAL DIFFRACTION TOMOGRAPHY; RED-BLOOD-CELLS; DIGITAL HOLOGRAPHY; PHASE MICROSCOPY; DYNAMICS; TRANSPARENT; BEHAVIOR; MASS;
D O I
10.3807/JOSK.2014.18.6.691
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Optical measurements of the morphological and biochemical imaging of phytoplankton are presented. Employing quantitative phase imaging techniques, 3-D refractive index maps and high-resolution 2-D quantitative phase images of individual live phytoplankton are simultaneously obtained without exogenous labeling agents. In addition, biochemical information of individual phytoplankton including volume, mass, and density of individual phytoplankton are also quantitatively obtained from the measured refractive index distributions. We expect the present method to become a powerful tool for the study of phytoplankton.
引用
收藏
页码:691 / 697
页数:7
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