Three-dimensional dynamics optical coherence tomography for tumor spheroid evaluation

被引：36

作者：

Abd El-Sadek, Ibrahim ^{[1
,2
]}

Miyazawa, Arata ^{[3
]}

Shen, Larina Tzu-Wei ^{[4
]}

Makita, Shuichi ^{[1
]}

Mukherjee, Pradipta ^{[1
]}

Lichtenegger, Antonia ^{[1
,5
]}

Matsusaka, Satoshi ^{[4
]}

Yasuno, Yoshiaki ^{[1
]}

机构：

[1] Univ Tsukuba, Computat Opt Grp, Tsukuba, Ibaraki 3058573, Japan

[2] Damietta Univ, Fac Sci, Dept Phys, Dumyat 34517, Egypt

[3] Sky Technol Inc, Tsukuba, Ibaraki 3050032, Japan

[4] Univ Tsukuba, Fac Med, Clin Res & Reg Innovat, Tsukuba, Ibaraki 3058575, Japan

[5] Med Univ Vienna, Ctr Med Phys & Biomed Engn, Wahringer Gurtel 18-20,4L, A-1090 Vienna, Austria

来源：

BIOMEDICAL OPTICS EXPRESS | 2021年 / 12卷 / 11期

基金：

日本科学技术振兴机构; 奥地利科学基金会; 日本学术振兴会;

关键词：

PRINCIPAL-COMPONENT-ANALYSIS; RETINAL-PIGMENT EPITHELIUM; ATTENUATION COEFFICIENTS; HUMAN SKIN; CONTRAST; SEGMENTATION; ELASTOGRAPHY; APOPTOSIS; MOTION; EYE;

D O I：

10.1364/BOE.440444

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a completely label-free three-dimensional (3D) optical coherence tomography (OCT)-based tissue dynamics imaging method for visualization and quantification of the metabolic and necrotic activities of tumor spheroid. Our method is based on a custom 3D scanning protocol that is designed to capture volumetric tissue dynamics tomography images only in a few tens of seconds. The method was applied to the evaluation of a tumor spheroid. The time-course viability alteration and anti-cancer drug response of the spheroid were visualized qualitatively and analyzed quantitatively. The similarity between the OCT-based dynamics images and fluorescence microscope images was also demonstrated.

引用

页码：6844 / 6863

页数：20

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