Comprehensive analysis of host gene expression in Autographa californica nucleopolyhedrovirus-infected Spodoptera frugiperda cells

被引:60
作者
Salem, Tamer Z. [1 ,4 ]
Zhang, Fengrui [1 ]
Xie, Yan [2 ]
Thiem, Suzanne M. [1 ,3 ]
机构
[1] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA
[2] Michigan State Univ, Ctr Stat Training & Consulting, E Lansing, MI 48824 USA
[3] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA
[4] Agr Res Ctr, Dept Microbial Mol Biol, AGERI, Giza 12619, Egypt
基金
美国国家卫生研究院;
关键词
Microarray; AcMNPV; Sf21; cells; Baculovirus; Host genes; qRT-PCR; Hsp; 70; DAVID; Transcriptome; NUCLEAR POLYHEDROSIS-VIRUS; CRYSTAL-STRUCTURE; INTRACELLULAR PROTEINS; FUNCTIONAL EXPRESSION; BACULOVIRUS INFECTION; RECEPTOR; CHANNEL; CHAPERONES; SYSTEMS; OVEREXPRESSION;
D O I
10.1016/j.virol.2011.01.006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) is the best-studied baculovirus and most commonly used virus vector for baculovirus expression vector systems. The effect of AcMNPV infection on host cells is incompletely understood. A microarray based on Spodoptera frugiperda ESTs was used to investigate the impact of AcMNPV on host gene expression in cultured S. frugiperda, Sf21 cells. Most host genes were down-regulated over the time course of infection, although a small number were up-regulated. The most highly up-regulated genes encoded heat shock protein 70s and several poorly characterized proteins. Regulated genes with the highest score identified by functional annotation clustering included primarily products required for protein expression and trafficking in the ER and golgi. All were significantly down-regulated by approximately 12 h post-infection. Microarray data were validated by qRT-PCR. This study provides the first comprehensive host transcriptome overview of Sf21 cells during AcMNPV infection. (c) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:167 / 178
页数:12
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