Identification of antioxidant peptides of hen egg-white lysozyme and evaluation of inhibition of lipid peroxidation and cytotoxicity in the Zebrafish model

被引：56

作者：

Carrillo, W. ^{[1
,2
]}

Gomez-Ruiz, J. A. ^{[1
]}

Miralles, B. ^{[1
]}

Ramos, M. ^{[1
]}

Barrio, D. ^{[3
]}

Recio, I. ^{[1
]}

机构：

[1] UAM, CSIC, CIAL, Inst Invest Ciencias Alimentac, Nicolas Cabrera 9, Madrid 28049, Spain

[2] Univ Tecn Ambato, Fac Ciencia & Ingn Alimentos, Ave Chasquis & Rio Payamino,Campus Huachi, Ambato 1801334, Ecuador

[3] Univ Nacl Rio Negro, Don Bosco & Leloir S-N, RA-8500 Viedma, Argentina

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2016年 / 242卷 / 10期

关键词：

Lysozyme; Antioxidant activity in Zebrafish larvae; Bioactive peptides; Hydrolyzate; Cation-exchange column; Toxicity in Zebrafish egg; ANTIBACTERIAL PEPTIDES; ANTIMICROBIAL PEPTIDES; PROTEINS; PURIFICATION; LACTOFERRIN; MECHANISMS; DIGESTION; EXPOSURE; PEPSIN;

D O I：

10.1007/s00217-016-2677-1

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Hen egg lysozyme was hydrolyzed with pepsin in situ on a cation-exchange column to isolate antioxidant peptides. The most cationic fraction was eluted with 1 M NaCl. Five positively charged peptides f(109-119) VAWRNRCKGTD, f(111-119) WRNRCKGTD, f(122-129) AWIRGCRL, f(123-129) WIRGCRL and f(124-129) IRGCRL were identified using tandem mass spectrometry. Using ORAC-FL , all five peptides presented antioxidant activity with values of (1970, 3123, 2743, 2393 and 0.313 A mu mol Trolox/A mu mol peptide), respectively. Using method TBARS in Zebrafish larvae, all five synthetic peptides were found to efficiently inhibit lipid peroxidation (36.8, 51.6, 55.56, 63.2, 61.0 % inhibition of lipid peroxidation), respectively. None of the five peptides were toxic in Zebrafish eggs and larvae at concentrations lower than 50 A mu g/ml. Concentrations higher than 50 A mu g/ml were toxic for both Zebrafish eggs and larvae.

引用

页码：1777 / 1785

页数：9

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