Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants

被引：9

作者：

Dorneles, E. M. S. ^{[1
]}

Araujo, M. S. S. ^{[2
]}

Teixeira-Carvalho, A. ^{[2
]}

Martins-Filho, O. A. ^{[2
]}

Lage, A. P. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Lab Bacteriol Aplicada, Dept Med Vet Prevent, Escola Vet, Belo Horizonte, MG, Brazil

[2] Fundacao Oswaldo Cruz, Ctr Pesquisas Rene Rachou, Lab Biomarcadores Diagnost & Monitoracao, Belo Horizonte, MG, Brazil

来源：

GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 01期

关键词：

Cytokines; Cross-reactivity; Human; Cattle; Goat; Sheep; T-CELLS; IFN-GAMMA; KINETICS; CATTLE; IL-10; ALPHA; BLOOD;

D O I：

10.4238/2015.February.3.1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eleven commercially available PE-labeled anti-human (IL-1-alpha, IL-6, IL-8, TNF-alpha, IL-17A, IL-5, IL-10, IL-12 and IL-13) and anti-mouse (IL-10, TNF-alpha) cytokine monoclonal antibodies (mAbs) were tested for cross-reactivity with cattle, goat, and sheep cytokines. Cross-reactivity was assessed by comparative analysis with the standard reactivity of the target species. Our data demonstrated that anti-human IL-1-alpha, IL-6, IL-8, IL-17A and IL-10 mAbs cross-react with all ruminant species tested. Anti-human IL-5 mAb showed a strong cross-reactivity with cattle and goat IL-5, while anti-human TNF-alpha mAb showed a selective cross-reactivity with goat TNF-alpha. No cross-reactivity with the ruminant cytokines was observed for anti-human IL-12 and IL-13

引用

页码：940 / 951

页数：12

共 19 条

[1]

Boehringer N, 1999, EUR CYTOKINE NETW, V10, P211

[2] Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species [J].