MiR-223-3p as a Novel MicroRNA Regulator of Expression of Voltage-Gated K+ Channel Kv4.2 in Acute Myocardial Infarction

被引：60

作者：

Liu, Xue ^{[1
]}

Zhang, Ying ^{[1
]}

Du, Weijie ^{[1
]}

Liang, Haihai ^{[1
]}

He, Hua ^{[1
]}

Zhang, Lu ^{[1
]}

Pan, Zhenwei ^{[1
]}

Li, Xuelian ^{[1
]}

Xu, Chaoqian ^{[1
]}

Zhou, Yuhong ^{[1
]}

Wang, Leimin ^{[1
]}

Qian, Ming ^{[1
]}

Liu, Tianyi ^{[1
]}

Yin, Hongli ^{[1
]}

Lu, Yanjie ^{[1
]}

Yang, Baofeng ^{[1
]}

Shan, Hongli ^{[1
]}

机构：

[1] Harbin Med Univ, Dept Pharmacol, State Prov Key Labs Biomed Pharmaceut China, Key Lab Cardiovasc Res,Minist Educ, Harbin 150086, Heilongjiang Pr, Peoples R China

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2016年 / 39卷 / 01期

基金：

中国国家自然科学基金;

关键词：

MicroRNA; miR-223-3p; Acute myocardial infarction; Arrhythmia; KCND2; Kv4.2; I-to; HUMAN ATRIAL MYOCYTES; RAT VENTRICULAR MYOCYTES; TRANSIENT OUTWARD; DOWN-REGULATION; CELL PROLIFERATION; CARDIAC FIBROSIS; PROTEIN; CURRENTS; REVEALS; HEART;

D O I：

10.1159/000445609

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: Acute myocardial infarction (AMI) is a devastating cardiovascular disease with a high rate of morbidity and mortality, partly due to enhanced arrhythmogenicity. MicroRNAs (miRNAs) have been shown to participate in the regulation of cardiac ion channels and the associated arrhythmias. The purpose of this study was to test our hypothesis that miR-223-3p contributes to the electrical disorders in AMI via modulating KCND2, the gene encoding voltage-gated channel Kv4.2 that carries transient outward K+ current I-to. Methods: AMI model was established in male Sprague-Dawley (SD) rats by left anterior descending artery (LAD) ligation. Evans blue and TTC staining was used to measure infarct area. I-to was recorded in isolated ventricular cardiomyocytes or cultured neonatal rat ventricular cells (NRVCs) by whole-cell patch-clamp techniques. Western blot analysis was employed to detect the protein level of Kv4.2 and real-time RT-PCR to determine the transcript level of miR-223-3p. Luciferase assay was used to examine the interaction between miR-223-3p and KCND2 in cultured NRVCs. Results: Expression of miR-223-3p was remarkably upregulated in AMI relative to sham control rats. On the contrary, the protein level of Kv4.2 and I-to density were significantly decreased in AMI. Consistently, transfection of miR-223-3p mimic markedly reduced Kv4.2 protein level and Ito current in cultured NRVCs. Co-transfection of AMO-223-3p (an antisense inhibitor of miR-223-3p) reversed the repressive effect of miR-223-3p. Luciferase assay showed that miR-223-3p, but not the negative control, substantially suppressed the luciferase activity, confirming the direct binding of miR-223-3p to the seed site within the KCND2 sequence. Finally, direct intramuscular injection of AMO-223-3p into the ischemic myocardium to knockdown endogenous miR-223-3p decreased the propensity of ischemic arrhythmias. Conclusions: Upregulation of miR-223-3p in AMI repressed the expression of KCND2/Kv4.2 resulting in reduction of I-to density that can cause APD prolongation and promote arrhythmias in AMI, and therefore knockdown of endogenous miR-223-3p might be considered a new approach for antiarrhythmic therapy of ischemic arrhythmias. (C) 2016 The Author(s) Published by S. Karger AG, Basel

引用

页码：102 / 114

页数：13

共 53 条

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