Fabrication of a Urea Biosensor for Real-Time Dynamic Fluid Measurement

被引：26

作者：

Kim, Kyunghee ^{[1
,2
,3
]}

Lee, Jeongeun ^{[1
,2
,3
]}

Moon, Bo Mi ^{[4
]}

Seo, Ye Been ^{[4
]}

Park, Chan Hum ^{[4
]}

Park, Min ^{[1
,2
,3
]}

Sung, Gun Yong ^{[1
,2
,3
]}

机构：

[1] Hallym Univ, Cooperat Course Nanomed Device Engn, Chunchon 24252, South Korea

[2] Hallym Univ, Dept Mat Sci & Engn, Chunchon 24252, South Korea

[3] Hallym Univ, Integrat Mat Res Inst, Chunchon 24252, South Korea

[4] Hallym Univ, Coll Med, Nanobio Regenerat Med Inst, Chunchon 24252, South Korea

来源：

SENSORS | 2018年 / 18卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

urea biosensor; silk fibroin; amination; flow conditions; real-time monitoring; AMPEROMETRIC AMMONIUM ION; DIALYSIS THERAPY; ELECTRODE; CARBON; POLYANILINE; ACID; SENSOR; KOREA;

D O I：

10.3390/s18082607

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this study, a portable urea sensor that monitors the urea concentration in flow conditions was fabricated. We propose an electrochemical sensor that continually measures the urea concentration of samples flowing through it at a constant flow rate in real time. For the electrochemical sensing, a porous silk fibroin membrane with immobilized urease was mounted in a polydimethylsiloxane (PDMS) sensor housing. The fabricated urea sensor elicited linear current-concentration characteristics in the clinically significant concentration range (0.1-20 mM) based on peritoneal dialysis. The sensor maintained the linear current-concentration characteristics during operation in flow conditions.

引用

页数：11

共 35 条

[1] Highly stable urea sensor based on ZnO nanorods directly grown on Ag/glass electrodes
Ahmad, Rafiq
Tripathy, Nirmalya
Hahn, Yoon-Bong
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2014, 194 : 290 - 295
[2] Potentiometric urea biosensor based on multi-walled carbon nanotubes (MWCNTs)/silica composite material
Ahuja, Tarushee
Kumar, D.
Singh, Nahar
Biradar, A. M.
Rajesh
[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2011, 31 (02): : 90 - 94
[3] Selective determination of urea using urease immobilized on ZnO nanowires
Ali, Syed M. Usman
Ibupoto, Zafar Hussain
Salman, Salah
Nur, Omer
Willander, Magnus
Danielsson, Bengt
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2011, 160 (01): : 637 - 643
[4] Amperometric ammonium ion and urea determination with enzyme-based probes
Bertocchi, P
Compagnone, D
Palleschi, G
[J]. BIOSENSORS & BIOELECTRONICS, 1996, 11 (1-2) : 1 - 10
[5] Recent developments in urea biosensors
Dhawan, Gunjan
Sumana, Gajjala
Malhotra, B. D.
[J]. BIOCHEMICAL ENGINEERING JOURNAL, 2009, 44 (01) : 42 - 52
[6] A disposable biosensor for urea determination in blood based on an ammonium-sensitive transducer
Eggenstein, C
Borchardt, M
Diekmann, C
Gründig, B
Dumschat, C
Cammann, K
Knoll, M
Spener, F
[J]. BIOSENSORS & BIOELECTRONICS, 1999, 14 (01) : 33 - 41
[7] A UREA-SPECIFIC ENZYME ELECTRODE
GUILBAULT, GG
MONTALVO, JG
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1969, 91 (08) : 2164 - +
[8] Haynes R., 2010, Surgery, V28, P525, DOI DOI 10.1016/J.MPSUR.2010.08.003
[9] Screening for Kidney Malfunction Using Injection Moulded Plastic Urinalysis Cartridge
Hong, Jongcheol
Sung, Gun Yong
[J]. BIOCHIP JOURNAL, 2016, 10 (02) : 111 - 117
[10] Renal replacement therapy in Korea, 2012
Jin, Dong Chan
Han, Jin Suk
[J]. KIDNEY RESEARCH AND CLINICAL PRACTICE, 2014, 33 (01) : 9 - 18

← 1 2 3 4 →