Intracavernous Pressure Recording in a Cavernous Nerve Injury Rat Model

被引：3
作者：
Li, Zitaiyu [1 ,2 ]
Yin, Yinghao [3 ]
He, Kancheng [1 ,2 ]
Ye, Kun [1 ,2 ]
Zhou, Jun [3 ]
Qi, Hao [1 ,2 ]
Zhou, Yihong [1 ,2 ]
Dai, Yingbo [1 ,2 ]
Tang, Yuxin [1 ,2 ]
机构：
[1] Sun Yat Sen Univ, Dept Urol, Affiliated Hosp 5, Guangzhou, Peoples R China
[2] Sun Yat Sen Univ, Guangdong Prov Key Lab Biomed Imaging, Affiliated Hosp 5, Guangzhou, Peoples R China
[3] Cent South Univ, Dept Urol, Xiangya Hosp 3, Changsha, Peoples R China
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2021年 / 175期
基金：
中国国家自然科学基金;
关键词：
NITRIC-OXIDE SYNTHASE; ERECTILE DYSFUNCTION; HEMODYNAMICS; REGENERATION; NEUROTOMY; RECOVERY;
D O I：
10.3791/63024
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The bilateral cavernous nerve (CN) injury rat model has been extensively used to simulate clinical cavernous nerve injury associated with erectile dysfunction (ED) for evaluating the effect of clinical therapeutic methods. However, the methods of CN injury model construction are flawed and varied in the ED research field. It is CN crush injury that is the most commonly used method in recent years. This study aims to provide a detailed description of the procedure of bilateral CN injury rat model construction and measurement of intracavernous pressure (ICP) recording, providing a reliable and reproducible CN injury rat model. This work successfully developed the CN injury method of hemostat crush injury using a syringe needle as hard support and a hemostat with a rubber sleeve. Also, this method concludes that a voltage of 1.0 V, frequency of 20 Hz, and pulse-width of 5 ms are the optimized stimulation parameters for ICP recording in a bilateral CN injury rat model.
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页数：10
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