Overexpression of NUDT7, a candidate quantitative trait locus for pork color, downregulates heme biosynthesis in L6 myoblasts

被引：10

作者：

Taniguchi, Masaaki ^{[1
]}

Hayashi, Takeshi ^{[1
]}

Nii, Masahiro ^{[2
]}

Yamaguchi, Tomomi ^{[2
]}

Fujishima-Kanaya, Naoe ^{[3
]}

Awata, Takashi ^{[1
]}

Mikawa, Satoshi ^{[1
]}

机构：

[1] Natl Inst Agrobiol Sci, Anim Genome Res Unit, Tsukuba, Ibaraki 3050901, Japan

[2] Tokushima Agr Forestry & Fisheries Technol Suppor, Livestock Res Inst, Tokushima 7711310, Japan

[3] Soc Technoinnovat Agri Forestry & Fisheries STAFF, Res Div 2, Tsukuba, Ibaraki 3050854, Japan

来源：

MEAT SCIENCE | 2010年 / 86卷 / 03期

关键词：

Differentiation; Gene expression; Heme biosynthesis; Meat color; QTL; Swine; MESSENGER-RNA; MUSCLE-CELLS; MEAT QUALITY; EXPRESSION; MYOGLOBIN; GENE; PROTEIN-3; MYOD;

D O I：

10.1016/j.meatsci.2010.05.045

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

While testing a quantitative trait locus (QTL) for pork color in a cross population of pigs from the mating of Large White dams to a Japanese wild boar, our laboratory discovered a candidate gene (NUDT7) that might affect heme biosynthesis in porcine muscle. Therefore, this experiment was designed to test the effect of NUDT7 on heme biosynthesis in cultured myoblasts. Rat L6 myoblasts were transfected with a mammalian expression vector for pig NUDT7 immediately after the induction of cell differentiation, and samples were harvested at 2, 4, 6, and 8 days. Expression of exogenous NUDT7 mRNA was highest on day 4, when the heme content was substantially lower (P<0.01) than that of the control (14.2 vs. 63.9 pmol/10(5) cells). These results suggest that overexpression of pig NUDT7 may be associated with heme biosynthesis downregulation in skeletal muscle, which may partially explain differences in meat color among breeds of livestock. (C) 2010 The American Meat Science Association. Published by Elsevier Ltd. All rights reserved.

引用

页码：728 / 732

页数：5

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