Utilization of deoiled Jatropha curcas seed cake for production of xylanase from thermophilic Scytalidium thermophilum

被引：24

作者：

Joshi, Chetna ^{[1
]}

Khare, S. K. ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Enzyme & Microbial Biochem Lab, New Delhi 110016, India

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 02期

关键词：

Biodiesel; Deoiled Jatropha curcas seed cake; Solid-state fermentation; Xylanase; Scytalidium thermophilum; SOLID-STATE FERMENTATION; THERMOMYCES-LANUGINOSUS; THERMOASCUS-AURANTIACUS; DEGRADING ENZYMES; HYPER-PRODUCTION; OPTIMIZATION; BAGASSE; STRAW; PULP;

D O I：

10.1016/j.biortech.2010.08.070

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Jatropha curcas is a major biodiesel crop. Large amount of deoiled cake is generated as by-product during biodiesel production from its seeds. Deoiled J. curcas seed cake was assessed as substrate for the production of xylanase from thermophilic fungus Scytalidium thermophilum by solid-state fermentation. The seed cake was efficiently utilized by S. thermophilum for its growth during which it produced good amount of heat stable extracellular xylanase. The solid-state fermentation conditions were optimized for maximum xylanase production. Under the optimized conditions viz. deoiled seed cake supplemented with 1% oat-spelt xylan, adjusted to pH 9.0, moisture content 1:3 w/v, inoculated with 1 x 10(6) spores per 5 g cake and incubated at 45 degrees C. 1455 U xylanase/g deoiled seed cake was obtained. The xylanase was useful in biobleaching of paper pulp. Solid-state fermentation of deoiled cake appears a potentially viable approach for its effective utilization. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1722 / 1726

页数：5

共 30 条

[1] INTERLABORATORY TESTING OF METHODS FOR ASSAY OF XYLANASE ACTIVITY [J].

BAILEY, MJ ;

BIELY, P ;

POUTANEN, K .

JOURNAL OF BIOTECHNOLOGY, 1992, 23 (03) :257-270

[2] Xylanases from Aspergillus niger, Aspergillus niveus and Aspergillus ochraceus produced under solid-state fermentation and their application in cellulose pulp bleaching [J].

Betini, J. H. A. ;

Michelin, M. ;

Peixoto-Nogueira, S. C. ;

Jorge, J. A. ;

Terenzi, H. F. ;

Polizeli, M. L. T. M. .

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2009, 32 (06) :819-824

[3] Kinetics of the solid state fermentation of sugarcane bagasse by Thermoascus aurantiacus for the production of xylanase [J].

dos Santos, E ;

Piovan, T ;

Roberto, IC ;

Milagres, AMF .

BIOTECHNOLOGY LETTERS, 2003, 25 (01) :13-16

[4] Optimization of Xylanase Production by Thermomyces lanuginosus in Solid State Fermentation [J].

Gaffney, Mark ;

Doyle, Sean ;

Murphy, Richard .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2009, 73 (12) :2640-2644

[5] Immobilization of xylan-degrading enzymes from Scytalidium thermophilum on Eudragit L-100 [J].

Gaur, R ;

Lata ;

Khare, SK .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2005, 21 (6-7) :1123-1128

[6] Characterization and stability of proteases from Penicillium sp produced by solid-state fermentation [J].

Germano, S ;

Pandey, A ;

Osaku, CA ;

Rocha, SN ;

Soccol, CR .

ENZYME AND MICROBIAL TECHNOLOGY, 2003, 32 (02) :246-251

[7] MEASUREMENT OF CELLULASE ACTIVITIES [J].

GHOSE, TK .

PURE AND APPLIED CHEMISTRY, 1987, 59 (02) :257-268

[8] Studies on the solid-state production of thermostable endoxylanases from Thermoascus aurantiacus:: Characterization of two isozymes [J].

Kalogeris, E ;

Christakopoulos, P ;

Kekos, D ;

Macris, BJ .

JOURNAL OF BIOTECHNOLOGY, 1998, 60 (03) :155-163

[9] Xylanase production by the thermophilic mold Humicola lanuginosa in solid-state fermentation [J].

Kamra, P ;

Satyanarayana, T .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2004, 119 (02) :145-157

[10] Extra-cellular L-glutaminase production by Zygosaccharomyces rouxii under solid-state fermentation [J].

Kashyap, P ;

Sabu, A ;

Pandey, A ;

Szakacs, G ;

Soccol, CR .

PROCESS BIOCHEMISTRY, 2002, 38 (03) :307-312

← 1 2 3 →