Basic aspects of absorption and fluorescence spectroscopy and resonance energy transfer methods

被引:11
作者
Shanker, Natasha [1 ]
Bane, Susan L. [1 ]
机构
[1] SUNY Binghamton, Dept Chem, Binghamton, NY 13902 USA
来源
BIOPHYSICAL TOOLS FOR BIOLOGISTS: VOL 1 IN VITRO TECHNIQUES | 2008年 / 84卷
关键词
D O I
10.1016/S0091-679X(07)84008-8
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Absorption and fluorescence spectroscopy are the heart of life science laboratory techniques. These two spectroscopic tools are sensitive, are easy to use, and can provide a wide range of information. The goal of this chapter is to familiarize the reader with some basic principles and applications of absorption and steady-state fluorescence spectroscopy. Selected applications are illustrated with examples.
引用
收藏
页码:213 / 242
页数:30
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