Brahma-related gene 1 ameliorates the neuronal apoptosis and oxidative stress induced by oxygen-glucose deprivation/reoxygenation through activation of Nrf2/HO-1 signaling

被引：22
作者：
Li, Feng [1 ]
Liang, Jing [2 ]
Tang, Dongfang [3 ]
机构：
[1] Xi An Jiao Tong Univ, Affiliated Hosp 1, Anesthesiol Dept, 277 West Yanta Rd, Xian 710061, Shaanxi, Peoples R China
[2] Xi An Jiao Tong Univ, Affiliated Hosp, Shaanxi Prov Tumor Hosp, Radiotherapy Dept,Med Coll, Xian 710061, Shaanxi, Peoples R China
[3] Fuwai Cent China Cardiovasc Hosp, Neurosurg Dept, Zhengzhou 450000, Henan, Peoples R China
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2018年 / 108卷
关键词：
Brg1; Cerebral ischemia and reperfusion; HO-1; Nrf2; OGD/R; ISCHEMIA-REPERFUSION INJURY; CEREBRAL-ARTERY OCCLUSION; HEME OXYGENASE-1; RAT-BRAIN; MOLECULAR-MECHANISMS; COLORECTAL-CANCER; BRG1; INTERACTS; EXPRESSION; NRF2; PROLIFERATION;
D O I：
10.1016/j.biopha.2018.09.144
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Accumulating evidence suggests that brahma-related gene 1 (Brg1) is a critical regulator of cell apoptosis and oxidative stress in response to various insults; however, whether Brg1 regulates neuronal apoptosis and oxidative stress during cerebral ischemia/reperfusion injury remains unclear. This study aimed to investigate the expression, biological function, and regulatory mechanism of Brg1 in regulating neuronal apoptosis and oxidative stress induced by oxygen-glucose deprivation/reoxygenation (OGD/R), an in vitro cellular model of cerebral ischemia and reperfusion injury. The results showed that Brg1 expression was altered in response to OGD/R treatment. Overexpression of Brg-1 increased cell viability, attenuated apoptosis, and reduced reactive oxygen species (ROS) production in neurons, thus showing a protective effect against OGD/R-induced injury. In contrast, knockdown of Brg1 significantly inhibited cell viability, increased apoptosis, and promoted ROS production in neurons following OGD/R treatment. Moreover, these results showed that Brg1 promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and increased the expression of heme oxygenase-1 (HO-1). However, knockdown of Nrf2 or HO-1 significantly abrogated Brg1-mediated neuroprotective effects. Taken together, these results demonstrate that Brg1 ameliorates OGD/R-induced neuronal injury in vitro by promoting the activation of Nrf2/HO-1 signaling. The study highlights a potential role of Brg1 in regulating cerebral ischemia/reperfusion injury in vivo.
引用
收藏
页码：1216 / 1224
页数：9
相关论文
共 55 条
[1]   Heme oxygenase-1 gene therapy: Recent advances and therapeutic applications [J].
Abraham, Nader G. ;
Asija, Amit ;
Drummond, George ;
Peterson, Stephen .
CURRENT GENE THERAPY, 2007, 7 (02) :89-108
[2]   Culturing pyramidal neurons from the early postnatal mouse hippocampus and cortex [J].
Beaudoin, Gerard M. J., III ;
Lee, Seung-Hye ;
Singh, Dipika ;
Yuan, Yang ;
Ng, Yu-Gie ;
Reichardt, Louis F. ;
Arikkath, Jyothi .
NATURE PROTOCOLS, 2012, 7 (09) :1741-1754
[3]   The chromatin remodelling factor BRG1 is a novel binding partner of the tumor suppressor p16INK4a [J].
Becker, Therese M. ;
Haferkamp, Sebastian ;
Dijkstra, Menno K. ;
Scurr, Lyndee L. ;
Frausto, Monika ;
Diefenbach, Eve ;
Scolyer, Richard A. ;
Reisman, David N. ;
Mann, Graham J. ;
Kefford, Richard F. ;
Rizos, Helen .
MOLECULAR CANCER, 2009, 8
[4]   Targeting of SWI/SNF chromatin remodelling complexes to estrogen-responsive genes [J].
Belandia, B ;
Orford, RL ;
Hurst, HC ;
Parker, MG .
EMBO JOURNAL, 2002, 21 (15) :4094-4103
[5]   Up-regulation of Heme oxygenase-1 attenuates brain damage after cerebral ischemia via simultaneous inhibition of superoxide production and preservation of NO bioavailability [J].
Chao, Xiaodong D. ;
Ma, Yihui H. ;
Luo, Peng ;
Cao, Lei ;
Lau, Wayne Bond ;
Zhao, Baocheng C. ;
Han, Feng ;
Liu, Wei ;
Ning, Weidong D. ;
Su, Ning ;
Zhang, Lei ;
Zhu, Jie ;
Fei, Zhou ;
Qu, Yan .
EXPERIMENTAL NEUROLOGY, 2013, 239 :163-169
[6]   Hepatic SMARCA4 predicts HCC recurrence and promotes tumour cell proliferation by regulating SMAD6 expression [J].
Chen, Zhiao ;
Lu, Xinyuan ;
Jia, Deshui ;
Jing, Ying ;
Chen, Di ;
Wang, Qifeng ;
Zhao, Fangyu ;
Li, Jinjun ;
Yao, Ming ;
Cong, Wenming ;
He, Xianghuo .
CELL DEATH & DISEASE, 2018, 9
[7]   BRG1 regulation by miR-155 in human leukemia and lymphoma cell lines (Publication with Expression of Concern. See vol. 21, pg. 1581, 2019) [J].
Cuadros, M. ;
Sanchez-Martin, V. ;
Herrera, A. ;
Balinas, C. ;
Martin-Padron, J. ;
Boyero, L. ;
Peinado, P. ;
Medina, P. P. .
CLINICAL & TRANSLATIONAL ONCOLOGY, 2017, 19 (08) :1010-1017
[8]   Central nervous system-specific knockout of Brg1 causes growth retardation and neuronal degeneration [J].
Deng, Li ;
Li, Guibo ;
Rao, Benqiang ;
Li, Huashun .
BRAIN RESEARCH, 2015, 1622 :186-195
[9]   Posttreatment with 11-Keto-β-Boswellic Acid Ameliorates Cerebral Ischemia-Reperfusion Injury: Nrf2/HO-1 Pathway as a Potential Mechanism [J].
Ding, Yi ;
Chen, MinChun ;
Wang, MingMing ;
Li, Yuwen ;
Wen, AiDong .
MOLECULAR NEUROBIOLOGY, 2015, 52 (03) :1430-1439
[10]   Stroke [J].
Donnan, Geoffrey A. ;
Fisher, Marc ;
Macleod, Malcolm ;
Davis, Stephen M. .
LANCET, 2008, 371 (9624) :1612-1623
123456