Chlorella vulgaris in a heterotrophic bioprocess: Study of the lipid bioaccessibility and oxidative stability

被引：39
作者：
Canelli, Greta [1 ]
Neutsch, Lukas [2 ]
Carpine, Roberta [2 ]
Tevere, Sabrina [2 ]
Giuffrida, Francesca [3 ]
Rohfritsch, Zhen [3 ]
Dionisi, Fabiola [3 ]
Bolten, Christoph J. [3 ]
Mathys, Alexander [1 ]
机构：
[1] Swiss Fed Inst Technol, Inst Food Nutr & Hlth, Schmelzbergstr 9, CH-8092 Zurich, Switzerland
[2] ZHAW, Campus Gruental, CH-8820 Wadenswil, Switzerland
[3] Nestle Res Ctr, Route Jorat 57, CH-1000 Lausanne, Switzerland
来源：
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | 2020年 / 45卷
关键词：
PUFAs; Bioaccessibility; Oxidation; Chlorella vulgaris; Heterotrophic cultivation; N-3; LC-PUFA; ANTIOXIDANT ACTIVITY; CELL-WALL; MICROALGAE; ACID; CAPACITY; BIOMASS; ASSAY; FOOD; PHOSPHOLIPIDS;
D O I：
10.1016/j.algal.2019.101754
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Microalgal biomass is an emerging source of several health-related compounds, including polyunsaturated fatty acids. Herein, Chlorella vulgaris was cultivated heterotrophically in a 16-L stirred tank bioreactor. The lipid oxidative stability and lipid bioaccessibility of the biomass harvested during the exponential and stationary phases were evaluated. The biomass harvested during the stationary phase showed lower lipid oxidation than that harvested during the exponential phase, likely due to the higher content of antioxidants in the former. In both biomasses, the hexanal and propanal profiles showed only moderate increase over 12 weeks of storage at 40 degrees C, indicating good oxidative stability. Lipid bioaccessibility measured in an infant in vitro model was 0.66% +/- 0.16% and 2.41% +/- 0.61% for the biomass harvested during the exponential and late stationary phases, respectively. This study indicates that C. vulgaris biomass can be considered as a stable and nutritious (optimal omega 3:omega 6 profile) source of essential fatty acids. Our results suggested that regarding lipid stability and bioaccessibility, harvesting during stationary phase could be preferred choice. In general, treatment of the biomass to increase lipid bioaccessibility should be investigated.
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页数：9
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