Determination of protein adducts of organophosphorus nerve agents in blood plasma

被引：15

作者：

Koryagina, N. L. ^{[1
]}

Savel'eva, E. I. ^{[1
]}

Karakashev, G. V. ^{[1
]}

Babakov, V. N. ^{[1
]}

Dubrovskii, Ya. A. ^{[1
]}

Ukolova, E. S. ^{[1
]}

Khlebnikova, N. S. ^{[1
]}

Murashko, E. A. ^{[1
]}

Koneva, V. Yu. ^{[1
]}

Ukolov, A. I. ^{[1
]}

Kopeikin, V. A. ^{[1
]}

Radilov, A. S. ^{[1
]}

机构：

[1] Fed Med Biol Agcy Russia, Res Inst Hyg Occupat Pathol & Human Ecol, Pos Kuzmolovskii 188663, Leningrad Oblas, Russia

来源：

JOURNAL OF ANALYTICAL CHEMISTRY | 2016年 / 71卷 / 08期

关键词：

organophosphorus agents; markers of exposure; retrospective analysis; gas chromatography; HPLC; mass-spectrometric detection; blood plasma; MASS-SPECTROMETRY; HUMAN SERUM; RAT PLASMA; BUTYRYLCHOLINESTERASE; REACTIVATION; EXPOSURE; ACETYLCHOLINESTERASE; IDENTIFICATION; QUANTIFICATION; ALBUMIN;

D O I：

10.1134/S1061934816080086

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography-mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography-mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.

引用

页码：849 / 859

页数：11

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