Mesalamine-Mediated Amelioration of Experimental Colitis in Piglets Involves Gut Microbiota Modulation and Intestinal Immune Cell Infiltration

被引：7

作者：

Huang, Yonggang ^{[1
]}

Wu, Miaomiao ^{[1
]}

Xiao, Hao ^{[2
]}

Liu, Hongnan ^{[3
]}

Yang, Guan ^{[4
]}

机构：

[1] Hunan Agr Univ, Coll Anim Sci & Technol, Anim Nutr Genome & Germplasm Innovat Res Ctr, Changsha, Peoples R China

[2] Guangdong Acad Agr Sci, Inst Anim Sci, State Key Lab Livestock & Poultry Breeding, Guangzhou, Peoples R China

[3] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Changsha, Peoples R China

[4] City Univ Hong Kong, Dept Infect Dis & Publ Hlth, Hong Kong, Peoples R China

来源：

FRONTIERS IN IMMUNOLOGY | 2022年 / 13卷

基金：

中国国家自然科学基金;

关键词：

colitis; gut microbiota; immune cells; mesalamine; piglet; INFLAMMATORY-BOWEL-DISEASE; REGULATORY T-CELLS; ASSOCIATION; MECHANISMS;

D O I：

10.3389/fimmu.2022.883682

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mesalamine (MES), also known as 5-aminosalicylic acid, is effective in treating mild to moderate ulcerative colitis (UC). The mechanisms of its actions are not fully elucidated. The aim of this study was to investigate the effects of MES treatment on intestinal microbiota and immune system in an dextran sulfate sodium (DSS)-induced UC model in postweaning piglets. Eighteen weaned piglets were assigned randomly to the following treatments: control group (CON, distilled water), DSS group (DSS, 3% DSS), and MES group (MES, 3% DSS + 2 g/day MES). Our results showed that MES treatment alleviates DSS-induced colitis in piglets, as evidenced by a reduced diarrhea index score and increased average daily gain (P < 0.05). This is accompanied by decreased diamine oxidase activity, D-lactate level (P < 0.05), and attenuated mucosal damage. MES treatment also decreased the abundance of Methanogens and reduced colon CD11b(+) macrophage and CD3(+) T-cell infiltrations in piglets with DSS-induced colitis (P < 0.05). Collectively, these data indicate that MES treatment-mediated colitis protection may involve microbiota and immune cell alterations.

引用

页数：10

共 46 条

[1] Resident and pro-inflammatory macrophages in the colon represent alternative context-dependent fates of the same Ly6Chi monocyte precursors [J].

Bain, C. C. ;

Scott, C. L. ;

Uronen-Hansson, H. ;

Gudjonsson, S. ;

Jansson, O. ;

Grip, O. ;

Guilliams, M. ;

Malissen, B. ;

Agace, W. W. ;

Mowat, A. Mc I. .

MUCOSAL IMMUNOLOGY, 2013, 6 (03) :498-510

[2] Human intestinal pro-inflammatory CD11chighCCR2+CX3CR1+ macrophages, but not their tolerogenic CD11c-CCR2-CX3CR1- counterparts, are expanded in inflammatory bowel disease [J].

Bernardo, D. ;

Marin, A. C. ;

Fernandez-Tome, S. ;

Montalban-Arques, A. ;

Carrasco, A. ;

Tristan, E. ;

Ortega-Moreno, L. ;

Mora-Gutierrez, I. ;

Diaz-Guerra, A. ;

Caminero-Fernandez, R. ;

Miranda, P. ;

Casals, F. ;

Caldas, M. ;

Jimenez, M. ;

Casabona, S. ;

De la Morena, F. ;

Esteve, M. ;

Santander, C. ;

Chaparro, M. ;

Gisbert, J. P. .

MUCOSAL IMMUNOLOGY, 2018, 11 (04) :1114-1126

[3]

Bernstein Charles N, 2017, Inflamm Intest Dis, V2, P116, DOI 10.1159/000481401

[4] Nitrated oleic acid up-regulates PPARγ and attenuates experimental inflammatory bowel disease [J].

Borniquel, Sara ;

Jansson, Emmelie A. ;

Cole, Marsha P. ;

Freeman, Bruce A. ;

Lundberg, Jon O. .

FREE RADICAL BIOLOGY AND MEDICINE, 2010, 48 (04) :499-505

[5] Microbiotas from Humans with Inflammatory Bowel Disease Alter the Balance of Gut Th17 and RORγt+ Regulatory T Cells and Exacerbate Colitis in Mice [J].

Britton, Graham J. ;

Contijoch, Eduardo J. ;

Mogno, Ilaria ;

Vennaro, Olivia H. ;

Llewellyn, Sean R. ;

Ng, Ruby ;

Li, Zhihua ;

Mortha, Arthur ;

Merad, Miriam ;

Das, Anuk ;

Gevers, Dirk ;

McGovern, Dermot P. B. ;

Singh, Namita ;

Braun, Jonathan ;

Jacobs, Jonathan P. ;

Clemente, Jose C. ;

Grinspan, Ari ;

Sands, Bruce E. ;

Colombel, Jean-Frederic ;

Dubinsky, Marla C. ;

Faith, Jeremiah J. .

IMMUNITY, 2019, 50 (01) :212-+

[6] Diagnostic and Clinical Significance of Serum Levels of D-Lactate and Diamine Oxidase in Patients with Crohn's Disease [J].

Cai, Jierui ;

Chen, Hong ;

Weng, Meiling ;

Jiang, Shuyu ;

Gao, Jie .

GASTROENTEROLOGY RESEARCH AND PRACTICE, 2019, 2019

[7] Gut Microbiota-driven Drug Metabolism in Inflammatory Bowel Disease [J].

Crouwel, Femke ;

Buiter, Hans J. C. ;

de Boer, Nanne K. .

JOURNAL OF CROHNS & COLITIS, 2021, 15 (02) :307-315

[8] The anti-inflammatory drug mesalamine targets bacterial polyphosphate accumulation [J].

Dahl, Jan-Ulrik ;

Gray, Michael J. ;

Bazopoulou, Daphne ;

Beaufay, Francois ;

Lempart, Justine ;

Koenigsknecht, Mark J. ;

Wang, Ying ;

Baker, Jason R. ;

Hasler, William L. ;

Young, Vincent B. ;

Sun, Duxin ;

Jakob, Ursula .

NATURE MICROBIOLOGY, 2017, 2 (04)

[9] Genome-wide association study implicates immune activation of multiple integrin genes in inflammatory bowel disease [J].

de lange, Katrina M. ;

Moutsianas, Loukas ;

Lee, James C. ;

Lamb, Christopher A. ;

Luo, Yang ;

Kennedy, Nicholas A. ;

Jostins, Luke ;

Rice, Daniel L. ;

Gutierrez-Achuryl, Javier ;

Ji, Sun-Gou ;

Heap, Graham ;

Nimmo, Elaine R. ;

Edwards, Cathryn ;

Henderson, Paul ;

Mowat, Craig ;

Sanderson, Jeremy ;

Satsangi, Jack ;

Simmons, Alison ;

Wilson, David C. ;

Tremelling, Mark ;

Hart, Ailsa ;

Mathew, Christopher G. ;

Newman, William G. ;

Parkes, Miles ;

Lees, Charlie W. ;

Uhlig, Holm ;

Hawkey, Chris ;

Prescott, Natalie J. ;

Ahmad, Tariq ;

Mansfield, John C. ;

Anderson, Carl A. ;

Barrett, Jeffrey C. .

NATURE GENETICS, 2017, 49 (02) :256-261

[10] Probiotics and prebiotics in ulcerative colitis [J].

Derikx, Lauranne A. A. P. ;

Dieleman, Levinus A. ;

Hoentjen, Frank .

BEST PRACTICE & RESEARCH CLINICAL GASTROENTEROLOGY, 2016, 30 (01) :55-71

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