FOXC2-AS1 stabilizes FOXC2 mRNA via association with NSUN2 in gastric cancer cells

被引：31
作者：
Yan, Jijun [1 ]
Liu, Juntao [1 ]
Huang, Zhengbin [1 ]
Huang, Wenwei [1 ]
Lv, Jianfa [1 ]
机构：
[1] Hanchuan Peoples Hosp, Dept Gen Surg, Hanchuan 431600, Hubei, Peoples R China
来源：
HUMAN CELL | 2021年 / 34卷 / 06期
关键词：
RNA stability; m(5)C modification; NSUN2; YBX1; NONCODING RNAS; EXPRESSION; PROLIFERATION; TUMORIGENESIS; ANGIOGENESIS; METASTASIS; RESISTANCE; SURVIVAL; EMT;
D O I：
10.1007/s13577-021-00583-3
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Long noncoding RNA (lncRNA) FOXC2-AS1 has been reported to act as an oncogene in multiple human cancers. However, the clinical significance, functional role and underlying mechanism of FOXC2-AS1 in gastric cancer (GC) remains largely unknown. Here, we found that FOXC2-AS1 expression was significantly elevated in GC tissues and cells, and overexpression of FOXC2-AS1 indicated advanced TNM stage and shorter overall survival in GC patients. Functionally, knockdown of FOXC2-AS1 attenuated the proliferation, migration and invasion of GC cells, whereas overexpression of FOXC2-AS1 showed the opposite effects. Further investigation revealed that FOXC2-AS1 interacted with FOXC2 mRNA and repressed its degradation. FOXC2-AS1 recruited RNA methyltransferase NSUN2 to FOXC2 mRNA, increasing its m(5)C level and association with YBX1. Taken together, our findings suggested that FOXC2-AS1 acted as an oncogenic lncRNA by stabilizing FOXC2 mRNA in an m(5)C-dependent manner, which may provide a novel therapeutic target for GC.
引用
收藏
页码：1755 / 1764
页数：10
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