Nanocomposite based on gold nanoparticles and carboxymethyl cellulose: Synthesis, characterization, antimicrobial, and anticancer activities

被引:101
作者
Doghish, Ahmed S. [1 ,2 ]
Hashem, Amr H. [3 ]
Shehabeldine, Amr M. [3 ]
Sallam, Al-Aliaa M. [4 ]
El-Sayyad, Gharieb S. [5 ,6 ]
Salem, Salem S. [3 ]
机构
[1] Badr Univ Cairo BUC, Dept Biochem, Fac Pharm, Badr City 11829, Cairo, Egypt
[2] Al Azhar Univ, Fac Pharm Boys, Biochem & Mol Biol Dept, Nasr City 11231, Cairo, Egypt
[3] Al Azhar Univ, Dept Bot & Microbiol, Fac Sci, Nasr City 11884, Cairo, Egypt
[4] Ain Shams Univ, Dept Biochem, Fac Pharm, Abbasiya 11566, Cairo, Egypt
[5] Galala Univ, Dept Microbiol & Immunol, Fac Pharm, Galal City, Suez, Egypt
[6] Egyptian Atom Energy Author EAEA, Drug Radiat Res Dept, Natl Ctr Radiat Res & Technol NCRRT, Drug Microbiol Lab, Cairo, Egypt
关键词
Gold nanoparticles; Carboxy methyl cellulose; Antimicrobial activity; Antifungal; Anticancer; BIOMEDICAL APPLICATIONS; INDUCE APOPTOSIS; GRAPHENE OXIDE; CANCER; DESIGN; ANTIBIOFILM; STABILITY; DELIVERY; ASSAY;
D O I
10.1016/j.jddst.2022.103874
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The current study fabricated a nanocomposite based on gold nanoparticles and carboxy methyl cellulose (CMC-AuNPs). The prepared nanocomposite (CMC-AuNPs) was fully characterized using FTIR, EDX, SEM, XRD, mapping, zeta potential, DLS, and TEM. In the colloidal dispersion, spherical and dispersed CMC-AuNPs with a diameter range of 10-90 nm were found. CMC-AuNPs displayed modest antibacterial activity against Staphylococcus aureus and Bacillus cereus, with minimal inhibitory concentrations (MICs) value of 25 mu g/ml, while CMCAuNPs had higher antibacterial activity against Klebsiella oxytoca and Escherichia coli with MICs value 50 and 100 mu g/ml respectively. In addition, CMC-AuNPs displayed antifungal activities against C. albicans, A. terreus, A. niger, and A. fumigatus with inhibitory zones 20, 13, 23, and 26 mm respectively at 500 mu g/ml. The MCF-7 recorded the lowest IC50 values (2.56 +/- 0.19 mu g/mL) for CMC-AuNPs, suggesting a strong cytotoxic effect on breast cancer cells. The percentage of total apoptosis was in CMC-AuNPs treated cells (38.81 +/- 1.99%) compared to 0.61 +/- 0.022% for control. As a result of our findings, we believe that the anticancer function of these CMCAuNPs is attributable to the activation of necrosis and apoptosis in breast cancer cells via enhanced caspase-8 and -9 activity and decreased VEGFR-2 levels. In conclusion, CMC-AuNPs were successfully synthesized and exhibited antibacterial, antifungal, and anticancer activities, which can be used in the pharmaceutical and medical fields.
引用
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页数:10
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共 85 条
[1]   Recent advances in waste-recycled nanomaterials for biomedical applications: Waste-to-wealth [J].
Abd Elkodous, Mohamed ;
El-Husseiny, Hussein M. ;
El-Sayyad, Gharieb S. ;
Hashem, Amr Hosny ;
Doghish, Ahmed S. ;
Elfadil, Dounia ;
Radwan, Yasmine ;
El-Zeiny, Hayam M. ;
Bedair, Heba ;
Ikhdair, Osama A. ;
Hashim, Hisham ;
Salama, Ahmed M. ;
Alshater, Heba ;
Ali Ahmed, Ahmed ;
Elsayed, Mahmoud Gamal ;
Nagy, Maria ;
Ali, Nouran Y. ;
Elahmady, Maryam ;
Kamel, Ahmed M. ;
Abd Elkodous, Mahmoud ;
Maallem, Imene ;
Kaml, Maria B. Sh. ;
Nasser, Nayera ;
Nouh, Ahmed AlaaEldin ;
Safwat, Fatma M. ;
Alshal, Mai M. ;
Ahmed, Salma K. ;
Nagib, Taha ;
-sayed, Fatma M. El ;
Almahdi, Manal ;
Adla, Yahia ;
ElNashar, Noha T. ;
Hussien, Aya Misbah ;
Salih, Alaa S. ;
Mahmoud, Somaya Abdulbaset ;
Magdy, Shireen ;
Ahmed, Diana I. ;
Hassan, Fayrouz Mohamed Saeed ;
Edward, Nermin A. ;
Milad, Kirolos Said ;
Halasa, Shereen R. ;
Arafa, Mohamed M. ;
Hegazy, Abdullah ;
Kawamura, Go ;
Tan, Wai Kian ;
Matsuda, Atsunori .
NANOTECHNOLOGY REVIEWS, 2021, 10 (01) :1662-1739
[2]   Structural, thermal and electrical studies of polyethylene oxide/starch blend containing green synthesized gold nanoparticles [J].
Abdelghany, A. M. ;
Oraby, A. H. ;
Asnag, G. M. .
JOURNAL OF MOLECULAR STRUCTURE, 2019, 1180 :15-25
[3]   Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility [J].
Abu-Elghait, Mohammed ;
Hasanin, Mohamed ;
Hashem, Amr Hosny ;
Salem, Salem S. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2021, 175 :294-303
[4]   Ecofriendly synthesis of silver nanoparticles using Kei-apple (Dovyalis caffra) fruit and their efficacy against cancer cells and clinical pathogenic microorganisms [J].
Al-Rajhi, Aisha M. H. ;
Salem, Salem S. ;
Alharbi, Asmaa A. ;
Abdelghany, T. M. .
ARABIAN JOURNAL OF CHEMISTRY, 2022, 15 (07)
[5]  
Al-Zahrani F.A.M., 2022, SYNTHESIS AG FE2O3 N
[6]   Green Synthesis and Antibacterial Activity of Ag/Fe2O3 Nanocomposite Using Buddleja lindleyana Extract [J].
Al-Zahrani, Fatimah A. M. ;
Salem, Salem S. ;
Al-Ghamdi, Huda A. ;
Nhari, Laila M. ;
Lin, Long ;
El-Shishtawy, Reda M. .
BIOENGINEERING-BASEL, 2022, 9 (09)
[7]   Green biosynthesis of titanium dioxide quantum dots using watermelon peel waste: antimicrobial, antioxidant, and anticancer activities [J].
Ali, Omar M. ;
Hasanin, Mohamed S. ;
Suleiman, Waleed B. ;
Helal, Eman El-Husseiny ;
Hashem, Amr H. .
BIOMASS CONVERSION AND BIOREFINERY, 2024, 14 (05) :6987-6998
[8]   Folic Acid-Functionalized, Condensed Magnetic Nanoparticles for Targeted Delivery of Doxorubicin to Tumor Cancer Cells Overexpressing the Folate Receptor [J].
Angelopoulou, Athina ;
Kolokithas-Ntoukas, Argiris ;
Fytas, Christos ;
Avgoustakis, Konstantinos .
ACS OMEGA, 2019, 4 (26) :22214-22227
[9]   Gold nanoparticles: opportunities and challenges in nanomedicine [J].
Arvizo, Rochelle ;
Bhattacharya, Resham ;
Mukherjee, Priyabrata .
EXPERT OPINION ON DRUG DELIVERY, 2010, 7 (06) :753-763
[10]   Dual Hyaluronic Acid and Folic Acid Targeting pH-Sensitive Multifunctional 2DG@DCA@MgO-Nano-Core-Shell-Radiosensitizer for Breast Cancer Therapy [J].
Askar, Mostafa A. ;
Thabet, Noura M. ;
El-Sayyad, Gharieb S. ;
El-Batal, Ahmed, I ;
Abd Elkodous, Mohamed ;
El Shawi, Omama E. ;
Helal, Hamed ;
Abdel-Rafei, Mohamed K. .
CANCERS, 2021, 13 (21)