Insulinotropic nucleobindin-2/nesfatin-1 is dynamically expressed in the haemochorial mouse and human placenta

被引：9

作者：

Legg-St Pierre, Crystalyn B. ^{[1
,2
]}

Mackova, Martina ^{[3
]}

Miskiewicz, Ewa I. ^{[1
,2
]}

Hemmings, Denise G. ^{[3
]}

Unniappan, Suraj ^{[1
,2
]}

MacPhee, Daniel J. ^{[1
,2
,4
]}

机构：

[1] Univ Saskatchewan, Western Coll Vet Med, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B4, Canada

[2] Univ Saskatchewan, Reprod Hlth Res Grp 1, Saskatoon, SK S7N 5B4, Canada

[3] Univ Alberta, Dept Obstet & Gynecol, Fac Med & Dent, Heritage Med Res Ctr 227, Edmonton, AB T6G 2S2, Canada

[4] Mem Univ Newfoundland, Fac Med, Div Biomed Sci, St John, NF A1B 3V6, Canada

来源：

REPRODUCTION FERTILITY AND DEVELOPMENT | 2018年 / 30卷 / 03期

基金：

加拿大健康研究院; 加拿大创新基金会;

关键词：

labyrinth; syncytiotrophoblast; trophoblast giant cells; CORD BLOOD NESFATIN-1; TROPHOBLAST DIFFERENTIATION; CELL; NUCB2/NESFATIN-1; SECRETION; MURINE; GROWTH; IDENTIFICATION; IMPLANTATION; ANGIOGENESIS;

D O I：

10.1071/RD16486

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The placenta is the physiological bridge between mother and fetus and has life-sustaining functions during pregnancy, including metabolic regulation, fetal protection and hormone secretion. Nucleobindin-2 (NUCB2) is a calcium- and DNA-binding protein and precursor of nesfatin-1, a signalling peptide with multiple functions, including regulation of energy homeostasis and glucose transport. These are also key functions of the placenta, yet NUCB2/nesfatin-1 expression has never been comprehensively studied in this organ. In the present study, mouse placental samples from Embryonic Day (E) 7.5 to E17.5 and human chorionic villi from the first and second trimester, as well as term pregnancy, were analysed for NUCB2/nesfatin-1 expression by immunohistochemistry with an antiserum that recognised both NUCB2 and nesfatin-1. From E7.5 to E9.5, NUCB2/nesfatin-1 was expressed in the ectoplacental cone, then parietal trophoblast giant cells and early spongiotrophoblast. At E10.5-12.5, NUCB2/nesfatin-1 expression became detectable in the developing labyrinth. From E12.5 and onwards, NUCB2/nesfatin-1 was expressed in the glycogen trophoblast cells, as well as highly expressed in syncytiotrophoblast, sinusoidal trophoblast giant cells and fetal capillary endothelial cells of the labyrinth. In all trimesters of human pregnancy, NUCB2/nesfatin-1 was highly expressed in syncytiotrophoblast. In addition, there was a significant increase in NUCB2 expression in human primary trophoblast cells induced to syncytialise. Thus, the haemochorial mammalian placenta is a novel source of NUCB2/nesfatin-1 and likely a site of its action, with potential roles in glucose homeostasis and/or nutrient sensing.

引用

页码：519 / 532

页数：14