Messenger RNA interactions in the decoding center control the rate of translocation

被引：39

作者：

Khade, Prashant K. ^{[1
]}

Joseph, Simpson ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2011年 / 18卷 / 11期

基金：

美国国家科学基金会;

关键词：

RIBOSOMAL-SUBUNIT; STATE; VIOMYCIN; HELIX; STEP;

D O I：

10.1038/nsmb.2140

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During protein synthesis, mRNA and tRNAs are iteratively translocated by the ribosome. Precisely what molecular event is rate limiting for translocation is not known. Here we show that disruption of the interactions between the A-site codon and the ribosome accelerates translocation, suggesting that the release of the mRNA from the decoding center of the ribosome is the rate-limiting step of translocation. These results provide insight into the molecular mechanism of translocation.

引用

页码：1300 / 1302

页数：3

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