Curdlan-like exopolysaccharide production by Cellulomonas flavigena UNP3 during growth on hydrocarbon substrates

被引：10
作者：
Arli, Sushma Deepthi [1 ]
Trivedi, U. B. [1 ]
Patel, K. C. [1 ]
机构：
[1] Sardar Patel Univ, BRD Sch Biosci, Vallabh Vidyanagar 388120, Gujarat, India
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2011年 / 27卷 / 06期
关键词：
Biodegradation; Bioemulsifier; Cellulomonas flavigena UNP3; Cell surface hydrophobicity; Curdlan; Exopolysaccharide; Fourier transform infrared spectroscopy; Nuclear magnetic resonance spectroscopy; FAECALIS VAR MYXOGENES; COMMERCIAL PRODUCTION; POLYSACCHARIDE; MUTANT; BIOSURFACTANTS; MICROORGANISMS; PURIFICATION; BACTERIAL;
D O I：
10.1007/s11274-010-0593-2
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Cellulomonas flavigena UNP3, a natural isolate from vegetable oil contaminated soil sample has been studied for growth associated exopolysaccharide (EPS) production during growth on glucose, groundnut oil and naphthalene. The EPS showed matrix formation surrounding the cells during scanning electron microscopy. Cell surface hydrophobicity and emulsifying activity studies confirmed the role of EPS as bioemulsifier. Emulsifying activity was found to increase with time (0.2 U/mg for 10 min to 0.27 U/mg for 30 min). Emulsification index, E-24 value increased with the increase in EPS concentration. Degradation of polyaromatic hydrocarbons was confirmed using gas chromatography analysis. FTIR analysis showed presence of characteristic absorbance at 895.10 cm(-1) for beta-configuration of glucan. NMR studies also revealed EPS produced by C. flavigena UNP3 as a linear beta-1, 3-d-glucan, and a curdlan like polysaccharide.
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页码：1415 / 1422
页数：8
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