Structure of stewartan, the capsular exopolysaccharide from the corn pathogen Erwinia stewartii

被引：29

作者：

Nimtz, M

Mort, A

Wray, V

Domke, T

Zhang, YX

Coplin, DL

Geider, K

机构：

[1] OKLAHOMA STATE UNIV, STILLWATER, OK 74078 USA

[2] MAX PLANCK INST MED RES, D-69120 HEIDELBERG, GERMANY

[3] OHIO STATE UNIV, DEPT PLANT PATHOL, COLUMBUS, OH 43210 USA

来源：

CARBOHYDRATE RESEARCH | 1996年 / 288卷

关键词：

exopolysaccharide; structure; Erwinia stewartii; Stewart's wilt; blight of corn;

D O I：

10.1016/S0008-6215(96)90797-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stewartan, the acidic exopolysaccharide of Erwinia stewartii, was purified from agar grown cells. To facilitate its structural analysis, chemical and enzymatic depolymerizations were carried out using hydrofluoric acid and E. amylovora phage phi-Ea1h, respectively. Structural characterization of the resulting oligosaccharides was performed by a combination of mass spectrometric and one- and two-dimensional (1D/2D) NMR spectroscopic techniques. A branched repeating unit with seven monosaccharide residues, all in their pyranose forms, was found: [GRAPHICS] (C) 1996 Elsevier Science Ltd.

引用

页码：189 / 201

页数：13

共 11 条

[1]

BERNHARD F, 1996, IN PRESS MICROBIOLOG

[2] MOLECULAR ANALYSIS OF THE AMS OPERON REQUIRED FOR EXOPOLYSACCHARIDE SYNTHESIS OF ERWINIA-AMYLOVORA [J].