Designed Three-Dimensional Collagen Scaffolds for Skin Tissue Regeneration

被引：67

作者：

Ahn, SeungHyun ^{[1
]}

Yoon, Hyeon ^{[1
]}

Kim, GeunHyung ^{[1
]}

Kim, YunYoung ^{[2
]}

Lee, SuHee ^{[2
]}

Chun, Wook ^{[3
]}

机构：

[1] Chosun Univ, Dept Mech Engn, Bio Nanofluid Lab, Kwangju 501759, South Korea

[2] Modern Cell & Tissue Technol Inc, Seoul, South Korea

[3] Hallym Univ, Coll Med, Hangang Sacred Heart Hosp, Dept Surg,Burn Ctr, Seoul, South Korea

来源：

TISSUE ENGINEERING PART C-METHODS | 2010年 / 16卷 / 05期

关键词：

POROUS SCAFFOLDS; EXTRACELLULAR-MATRIX; ARTIFICIAL SKIN; FABRICATION; REPAIR; SIZE;

D O I：

10.1089/ten.tec.2009.0511

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

One of the challenges in tissue engineering is the development of a reproducible three-dimensional (3D) scaffold to support cell migration and infiltration. As a dermal substitute, 3D collagen scaffolds with precisely controlled pore structures were fabricated using an innovative cryogenic dispenser system. The scaffolds were composed of perpendicular, highly porous collagen strands in successive layers. The fabricated scaffolds were evaluated in an in vitro keratinocyte/fibroblast coculture test. Fibroblasts were well dispersed within the scaffold, and keratinocytes had completely migrated through the well-designed pore structure and differentiated on top of the scaffold surface. The differentiated keratinocytes generated a stratum corneum in the 3D dispensed scaffolds, similar to that in normal skin tissue.

引用

页码：813 / 820

页数：8

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