Polarised light microscopy

被引：0

作者：

Torok, P ^{[1
]}

Higdon, PD ^{[1
]}

Wilson, T ^{[1
]}

机构：

[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

来源：

THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING V, PROCEEDINGS OF | 1998年 / 3261卷

关键词：

confocal microscopy; polarised light; vectorial theory; high aperture theory;

D O I：

10.1117/12.310539

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this work we discuss the role of polarisation in confocal microscopy. The effect of the optical system on light polarisation is analysed in the absence of a specimen. We also present numerical results on confocal microscopes imaging small dielectric scatterers and suggest an optimum pinhole size for crossed linear polars. Finally, imaging of large spherical scatterers and contrast optimisation of the optical system is discussed.

引用

页码：22 / 26

页数：5