A Sensor for Serotonin and Dopamine Detection in Cancer Cells Line Based on the Conducting Polymer-Pd Complex Composite

被引：21
作者：
Chung, Saeromi [1 ,2 ]
Akhtar, Mahmood H. [1 ,2 ]
Benboudiaf, A. [1 ,2 ]
Park, Deog-Su [2 ]
Shim, Yoon-Bo [1 ,2 ]
机构：
[1] Pusan Natl Univ, Dept Chem, Busan 46241, South Korea
[2] Pusan Natl Univ, Inst BioPhysio Sensor Technol, Busan 46241, South Korea
来源：
ELECTROANALYSIS | 2020年 / 32卷 / 03期
关键词：
Serotonin; Electrochemical sensor; Conducting polymer; Pd complex; Breast cancer cell; WHOLE-BLOOD; PLASMA;
D O I：
10.1002/elan.201900568
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An electrochemical sensor based on the conducting polymer composite with a palladium complex (Pd(C2H4N2S2)(2)) was developed for the detection of serotonin and dopamine simultaneously in the breast cancer cell and human plasma samples. The proposed sensor was fabricated using the Pd(C2H4N2S2)(2) complex-anchored poly2,2 : 5,2-terthiophene-3-(p-benzoic acid) (pTBA) layer on the AuNPs decorated reduced graphene oxide (AuNPs@rGO) substrate, which revealed the enhanced anodic current of the target species. The sensor probe was characterized by electrochemical and surface analysis methods. The experimental parameters affecting the sensor performance were optimized, in terms of AuNPs@rGO concentration, the number of electropolymerization cycle for pTBA, immobilization time of Pd(C2H4N2S2)(2), and pH. The dynamic ranges for serotonin and dopamine were obtained from 0.02 to 200 mu M, and from 0.1 to 200 mu M with the detection limit of 2.5, and 24.0 nM, respectively. The reliability of proposed sensor was evaluated using cancer cell lines for the clinical applications.
引用
收藏
页码：520 / 527
页数：8
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