Molecular marker screening of tomato, (solanum lycopersicum L.) Germplasm for root-knot nematodes (Meloidogyne species) resistance

被引：0

作者：

Danso, Y. ^{[1
]}

Akromah, R. ^{[2
]}

Osei, K. ^{[1
]}

机构：

[1] Crops Res Inst CSIR, Kumasi, Ghana

[2] Kwame Nkrumah Univ Sci & Technol, Dept Crop & Soil Sci, Kumasi, Ghana

来源：

AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 09期

关键词：

Germplasm; Meloidogyne incognita; molecular marker; resistance; Solanum lycopersicum; GENE; MI;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tomato is one of the crops in which genetic resistance has specially been effective against root-knot nematodes. In this study, molecular screening was done on some tomato germplasm to detect markers for the gene that confers resistance (Mi) with specific primer (Mi23/F//Mi23/R). The cultivars; VFNT, FLA 505-BL 1172, 2641A, "Adwoa Deede" and Terminator FI showed the marker for the homozygous resistant genotypes (Mi/Mi). The cultivars, Tima and 2644A showed both markers, corresponding to heterozygous resistant genotypes (Mi/mi). Twenty one (21) of the cultivars did not show any of the markers presumably due to non-specificity at the primer-binding sites. Five (5) heterozygous individuals were determined out of 6 resistant cultivars following the Hardy-Weinberg principle in population genetics.

引用

页码：1511 / 1515

页数：5

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[2]

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[3]

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[4]

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