TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro

被引：24
作者：
Castiglioni, Sara [1 ]
Romeo, Valentina [1 ]
Locatelli, Laura [1 ]
Cazzaniga, Alessandra [1 ]
Maier, Jeanette A. M. [1 ]
机构：
[1] Univ Milan, Dipartimento Sci Biomed & Clin L Sacco, I-20157 Milan, Italy
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
关键词：
STROMAL CELLS; MAGNESIUM; AUTOPHAGY; MINERALIZATION; INHIBITION; HOMEOSTASIS; RESISTANT; DELETION; RISE;
D O I：
10.1038/s41598-018-34324-8
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Mesenchymal stem cells are fundamental for bone formation and repair since they respond to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiation of human mesenchymal stem cells. TRPM7 and MagT1 are upregulated in osteogenic differentiation and silencing either one accelerates osteogenic differentiation, partly through the activation of autophagy. Intriguingly, similar results were obtained when the cells were cultured under magnesium deficient conditions. These results underpin the contribution of magnesium, TRPM7 and MagT1 to autophagy and osteoblastogenesis.
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页数：10
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