Discrimination of collagen in normal and pathological dermis through polarization second harmonic generation

被引：0

作者：

Su, Ping-Jung ^{[1
]}

Chen, Wei-Liang ^{[1
]}

Hong, Jin-Bon ^{[3
]}

Li, Tsung-Hsien ^{[1
]}

Wu, Ruei-, Jr. ^{[1
]}

Chou, Chen-Kuan ^{[1
]}

Lin, Sung-Jan ^{[2
,3
]}

Dong, Chen-Yuan ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Phys, 1 Sec 4th Roosevelt Rd, Taipei 106, Taiwan

[2] Natl Taiwan Univ, Inst Biomed Engn, Taipei 106, Taiwan

[3] Natl Taiwan Univ, Dept Dermatol, Taipei 106, Taiwan

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES X | 2010年 / 7569卷

关键词：

Dermatology; Second Harmonic Generation; Collagen; PATHOGENESIS; MULTIPHOTON; MYOSIN; TISSUE;

D O I：

10.1117/12.843370

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We used polarization-resolved, second harmonic generation (P-SHG) microscopy at single pixel resolution for medical diagnosis of pathological skin dermis, and found that P-SHG can be used to distinguish normal and dermal pathological conditions of keloid, morphea, and dermal elastolysis. We find that the histograms of the d(33)/d(31) ratio for the pathological skins to contain two peak values and to be wider than that of the normal case, suggesting that the pathological dermal collagen fibers tend to be more structurally heterogeneous. Our work demonstrates that pixel-resolved, second-order susceptibility microscopy is effective for detecting heterogeneity in spatial distribution of collagen fibers.

引用

页数：6

共 15 条

[1] Keloid pathogenesis and treatment [J].

Al-Attar, A ;

Mess, S ;

Thomassen, JM ;

Kauffman, CL ;

Davison, SP .

PLASTIC AND RECONSTRUCTIVE SURGERY, 2006, 117 (01) :286-300

[2] PENTOXIFYLLINE INHIBITS THE PROLIFERATION OF HUMAN FIBROBLASTS DERIVED FROM KELOID, SCLERODERMA AND MORPHEA SKIN AND THEIR PRODUCTION OF COLLAGEN, GLYCOSAMINOGLYCANS AND FIBRONECTIN [J].

BERMAN, B ;

DUNCAN, MR .

BRITISH JOURNAL OF DERMATOLOGY, 1990, 123 (03) :339-346

[3] Combination of multiphoton and reflective confocal imaging of cornea [J].

Chen, Wei-Liang ;

Sun, Yen ;

Lo, Wen ;

Tan, Hsin-Yuan ;

Dong, Chen-Yuan .

MICROSCOPY RESEARCH AND TECHNIQUE, 2008, 71 (02) :83-85

[4] Polarization ellipticity compensation in polarization second-harmonic generation microscopy without specimen rotation [J].

Chou, Chen-Kuan ;

Chen, Wei-Liang ;

Fwu, Peter Tramyeon ;

Lin, Sung-Jan ;

Lee, Hsuan-Shu ;

Dong, Chen-Yuan .

JOURNAL OF BIOMEDICAL OPTICS, 2008, 13 (01)

[5] Studies of x(2)/x(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy [J].

Chu, SW ;

Chen, SY ;

Chern, GW ;

Tsai, TH ;

Chen, YC ;

Lin, BL ;

Sun, CK .

BIOPHYSICAL JOURNAL, 2004, 86 (06) :3914-3922

[6] Nondestructive imaging of live human keloid and facial tissue using multiphoton microscopy [J].

Da Costa, Victor ;

Wei, Randy ;

Lim, Ryan ;

Sun, Chung-Ho ;

Brown, Jininiy J. ;

Wong, Brian J. -F. .

ARCHIVES OF FACIAL PLASTIC SURGERY, 2008, 10 (01) :38-43

[7] Second harmonic properties of tumor collagen: determining the structural relationship between reactive stroma and healthy stroma [J].

Han, Xiaoxing ;

Burke, Ryan M. ;

Zettel, Martha L. ;

Tang, Ping ;

Brown, Edward B. .

OPTICS EXPRESS, 2008, 16 (03) :1846-1859

[8] Acquired disorders of elastic tissue: Part II. Decreased elastic tissue [J].

Lewis, KG ;

Bercovitch, L ;

Dill, SW ;

Robinson-Bostom, L .

JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 2004, 51 (02) :165-185

[9] Mid-dermal elastolysis preceded by acute neutrophilic dermatosis [J].

Lewis, KG ;

Dill, SW ;

Wilkel, CS ;

Robinson-Bostom, L .

JOURNAL OF CUTANEOUS PATHOLOGY, 2004, 31 (01) :72-76

[10] Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres [J].

Plotnikov, SV ;

Millard, AC ;

Campagnola, PJ ;

Mohler, WA .

BIOPHYSICAL JOURNAL, 2006, 90 (02) :693-703

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