An Innovative Nanobody-Based Electrochemical Immunosensor Using Decorated Nylon Nanofibers for Point-of-Care Monitoring of Human Exposure to Pyrethroid Insecticides

被引：57
作者：
El-Moghazy, Ahmed Y. [1 ,2 ]
Huo, Jingqian [3 ,4 ,5 ]
Amaly, Noha [1 ,2 ]
Vasylieva, Natalia [3 ,4 ]
Hammock, Bruce D. [3 ,4 ]
Sun, Gang [1 ]
机构：
[1] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA
[2] City Sci Res & Technol Applicat SRTA City, Adv Technol & New Mat Res Inst, Polymer Mat Res Dept, New Borg El Arab City, Egypt
[3] Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA
[4] Univ Calif Davis, UCD Comprehens Canc Ctr, Davis, CA 95616 USA
[5] Agr Univ Hebei, Coll Plant Protect, Baoding 071001, Peoples R China
来源：
ACS APPLIED MATERIALS & INTERFACES | 2020年 / 12卷 / 05期
关键词：
nylon; nanofibers; electrochemical immunosensor; nanobody; pyrethroids; point-of-care; 3-PBA;
D O I：
10.1021/acsami.9b16193
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
A novel ultrasensitive nanobody-based electrochemical immunoassay was prepared for assessing human exposure to pyrethroid insecticides. 3-Phenoxybenzoic acid (3-PBA) is a common human urinary metabolite for numerous pyrethroids, which broadly served as a biomarker for following the human exposure to this pesticide group. The 3-PBA detection was via a direct competition for binding to alkaline phosphatase-embedded nanobodies between free 3-PBA and a 3-PBA-bovine serum albumin conjugate covalently immobilized onto citric acid-decorated nylon nanofibers, which were incorporated on a screen-printed electrode (SPE). Electrochemical impedance spectroscopy (EIS) was utilized to support the advantage of the employment of nanofibrous membranes and the success of the immunosensor assembly. The coupling between the nanofiber and nanobody technologies provided an ultrasensitive and selective immunosensor for 3-PBA detection in the range of 0.8 to 1000 pg mL(-1) with a detection limit of 0.64 pg mL(-1). Moreover, when the test for 3-PBA was applied to real samples, the established immunosensor proved to be a viable alternative to the conventional methods for 3-PBA detection in human urine even without sample cleanup. It showed excellent properties and stability over time.
引用
收藏
页码：6159 / 6168
页数：10
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