Rat Monoclonal Antibody Specific for MyoD

被引：3

作者：

Harada, Akihito ^{[2
]}

Ohkawa, Yasuyuki ^{[2
]}

Ao, Shinpei ^{[1
]}

Odawara, Jun ^{[2
]}

Okada, Seiji ^{[2
]}

Azuma, Masayuki ^{[1
]}

Nishiyama, Yuko ^{[2
]}

Nakamura, Mako ^{[2
]}

Tachibana, Taro ^{[1
]}

机构：

[1] Osaka City Univ, Dept Bioengn, Grad Sch Engn, Sumiyoshi Ku, Osaka 5588585, Japan

[2] Kyushu Univ, Fac Med, Dept Epigenet, SSP Stem Cell Unit, Fukuoka 812, Japan

来源：

HYBRIDOMA | 2010年 / 29卷 / 03期

关键词：

SKELETAL-MUSCLE; DNA-BINDING; REGULATORY FACTORS; MYOGENESIS; EXPRESSION; GENERATION; PROTEINS; COMPLEX; CELLS; MYF-5;

D O I：

10.1089/hyb.2009.0117

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.

引用

页码：255 / 258

页数：4

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