Fangchinoline protects against bone loss in OVX mice via inhibiting osteoclast formation, bone resorption and RANKL-induced signaling

被引：16

作者：

Zhou, Lin ^{[1
,2
]}

Hong, Guoju ^{[1
,3
]}

Li, Shangfu ^{[1
,4
]}

Liu, Qian ^{[1
,5
,6
]}

Song, Fangming ^{[1
,5
,6
]}

Zhao, Jinmin ^{[5
,6
]}

Yuan, Jinbo ^{[1
]}

Tickner, Jennifer ^{[1
]}

Xu, Jiake ^{[1
]}

机构：

[1] Univ Western Australia, Sch Biomed Sci, Perth, WA 6009, Australia

[2] Guangzhou Med Univ, Affiliated Hosp 5, Dept Endocrinol, Guangzhou 510700, Guangdong, Peoples R China

[3] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Orthoped Dept, Guangzhou 510006, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Affiliated Hosp 3, Dept Spine Surg, Guangzhou 510630, Guangdong, Peoples R China

[5] Guangxi Med Univ, Res Ctr Regenerat Med, Guangxi 530021, Peoples R China

[6] Guangxi Med Univ, Guangxi Key Lab Regenerat Med, Guangxi 530021, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES | 2020年 / 16卷 / 02期

基金：

英国医学研究理事会;

关键词：

fangchinoline; RANKL; osteoclast; NF-kappa B; NFATc1; MAPK; NF-KAPPA-B; RECEPTOR ACTIVATOR; RAT; DIFFERENTIATION; OSTEOPOROSIS;

D O I：

10.7150/ijbs.37162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Osteoporosis is a disease characterized by abnormally increased formation and function of osteoclasts. Anti-RANKL treatment using natural medicine is a potential therapy for osteoporosis. Here, we studied the effect of fangchinoline, which is extracted from the root of Stephania tetrandra S. Moore, on osteoclast formation and function. We found that fangchinoline inhibited osteoclastogenesis at doses of 0.5 and 1 mu M. In addition, we also examined the mechanism of the inhibitory effect of fangchinoline on osteoclasts. We found that fangchinoline down regulated NFATc1 activity and expression. However, fangchinoline did not affect IKB alpha degradation and MAPK pathways. In addition, we also found that fangchinoline could protect against bone loss in OVX mice. Taken together, fangchinoline may be a potential compound for osteoporosis.

引用

页码：309 / 319

页数：11

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