Electrospun PCL/PLA/HA Based Nanofibers as Scaffold for Osteoblast-Like Cells

被引：68

作者：

Fang, Rui ^{[1
,2
]}

Zhang, Enwei ^{[2
]}

Xu, Ling ^{[1
,2
]}

Wei, Shicheng ^{[2
,3
]}

机构：

[1] Peking Univ, Coll Engn, Beijing 100871, Peoples R China

[2] Peking Univ, Ctr Biomed Mat & Tissue Engn, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China

[3] Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2010年 / 10卷 / 11期

关键词：

PCL/PLA; HA; Osteoblast-Like Cell; Electrospun; Bone Tissue Engineering; HYDROXYAPATITE NANOPARTICLES; CALCIUM PHOSPHATES; TISSUE; PROLIFERATION; CRYSTALLINITY;

D O I：

10.1166/jnn.2010.2831

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Polycaprolactone (PCL), poly (lactic acid) (PLA) and hydroxyapatite (HA) are frequently used as materials for tissue engineering. In this study, PCL/PLA/HA nanofiber mats with different weight ratio were prepared using electrospinning. Their structure and morphology were studied by FTIR and FESEM. FTIR results demonstrated that the HA particles were successfully incorporated into the PCL/PLA nanofibers. The FESEM images showed that the surface of fibers became coarser with the introduction of HA nanoparticles into PCL/PLA system. Furthermore, the addition of HA led to the decreasing of fiber diameter. The average diameters of PCL/PLA/HA nanofiber were in the range of 300-600 nm, while that of PCL/PLA was 776 +/- 15.4 nm. The effect of nanofiber composition on the osteoblast-like MC3T3-E1 cell adhesion and proliferation were investigated as the preliminary biological evaluation of the scaffold. The MC3T3-E1 cell could be attached actively on all the scaffolds. The MU assay revealed that PCL/PLA/HA scaffold shows significantly higher cell proliferation than PCL/PLA scaffolds. After 15 days of culture, mineral particles on the surface of the cells was appeared on PCL/PLA/HA nanofibers while normal cell spreading morphology on PCL/PLA nanofibers. These results manifested that electrospun PCL/PLA/HA scaffolds could enhance bone regeneration, showing their marvelous prospect as scaffolds for bone tissue engineering.

引用

页码：7747 / 7751

页数：5

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