Regulation of nonsymbiotic and truncated hemoglobin genes of Lotus japonicus in plant organs and in response to nitric oxide and hormones

被引:63
作者
Bustos-Sanmamed, Pilar [1 ]
Tovar-Mendez, Alejandro [1 ]
Crespi, Martin [2 ]
Sato, Shusei [3 ]
Tabata, Satoshi [3 ]
Becana, Manuel [1 ]
机构
[1] Consejo Super Invest Cient, Dept Nutr Vegetal, Estac Expt Aula Dei, Zaragoza 50080, Spain
[2] CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France
[3] Kazusa DNA Res Inst, Chiba 2920818, Japan
关键词
hormones; legumes; nitric oxide; nonsymbiotic hemoglobins; root nodules; truncated hemoglobins; vascular bundles; NON-SYMBIOTIC HEMOGLOBIN; LEGUME ROOT-NODULES; ARABIDOPSIS-THALIANA; MEDICAGO-TRUNCATULA; NITROGEN-FIXATION; HYPOXIC STRESS; EXPRESSION; NITRATE; NO; GROWTH;
D O I
10.1111/j.1469-8137.2010.03527.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In legumes, symbiotic leghemoglobins facilitate oxygen diffusion to the bacteroids, but the roles of nonsymbiotic and truncated hemoglobins are largely unknown. Here the five hemoglobin genes of Lotus japonicus have been functionally characterized to gain insight into their regulatory mechanisms. Plants were exposed to nitric oxide donors, stressful conditions, and hormones. Gene expression profiling was determined by quantitative PCR, and gene activities were localized using in situ hybridization and promoter-reporter gene fusions. The LjGLB1-1, LjGLB2, and LjGLB3-1 mRNA expression levels were very high in nodules relative to other plant organs. The expression of these genes was localized in the vascular bundles, cortex, and infected tissue. LjGLB1-1 was the only gene induced by nitric oxide. Cytokinins caused nearly complete inactivation of LjGLB2 and LjGLB3-1 in nodules and induction of LjGLB1-1 in roots. Abscisic acid induced LjGLB1-1 in nodules and LjGLB1-2 and LjGLB2 in roots, whereas polyamines and jasmonic acid induced LjGLB1-1 only in roots. The enhanced expression of the three types of hemoglobins in nodules, the colocalization of gene activities in nodule and root tissues with high metabolic rates, and their distinct regulatory mechanisms point out complementary roles of hemoglobins and strongly support the hypothesis that LjGLB1-1, LjGLB2, and LjGLB3-1 are required for symbiosis.
引用
收藏
页码:765 / 776
页数:12
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