DNA barcoding is a useful tool for the identification of marine fishes from Japan

被引:122
作者
Zhang, Jun-Bin [1 ,2 ]
Hanner, Robert [2 ]
机构
[1] Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
[2] Univ Guelph, Dept Integrat Biol, Guelph, ON, Canada
关键词
DNA barcoding; Marine fishes; Japan; LIFE; REEF;
D O I
10.1016/j.bse.2010.12.017
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, 229 DNA sequences of cytochrome oxidase subunit I gene (COI) from 158 marine fishes of Japan were employed to test the efficacy of species identification by DNA barcoding. The average genetic distance was 60-fold higher between species than within species, as Kimura two parameter (K2P) genetic distances averaged 17.6% among congeners and only 0.3% among conspecifics. There were no overlaps between intraspecific and interspecific K2P distances, and all sequences formed species units in the neighbor-joining dendrogram. Hybridization phenomena in two species (Kyphosus vaigiensis and Pterocaesio digramma) were also detected through searches in Barcode of Life Data Systems (BOLD). DNA barcoding provides a new way for fish identification. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
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页码:31 / 42
页数:12
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