SNARE-protein-mediated disease resistance at the plant cell wall

被引:734
作者
Collins, NC
Thordal-Christensen, H
Lipka, V
Bau, S
Kombrink, E
Qiu, JL
Hückelhoven, R
Stein, M
Freialdenhoven, A
Somerville, SC
Schulze-Lefert, P [1 ]
机构
[1] Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany
[2] John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
[3] Riso Natl Lab, Plant Res Dept, DK-4000 Roskilde, Denmark
[4] Univ Giessen, Inst Phytopathol & Appl Zool, D-35392 Giessen, Germany
[5] Carnegie Inst Washington, Dept Plant Biol, Stanford, CA 94305 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1038/nature02076
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Failure of pathogenic fungi to breach the plant cell wall constitutes a major component of immunity of non-host plant species species outside the pathogen host range - and accounts for a proportion of aborted infection attempts on 'susceptible' host plants (basal resistance)(1-4). Neither form of penetration resistance is understood at the molecular level. We developed a screen for penetration ( pen) mutants of Arabidopsis, which are disabled in non-host penetration resistance against barley powdery mildew, Blumeria graminis f. sp. hordei, and we isolated the PEN1 gene. We also isolated barley ROR2 (ref. 2), which is required for basal penetration resistance against B. g. hordei. The genes encode functionally homologous syntaxins, demonstrating a mechanistic link between non-host resistance and basal penetration resistance in monocotyledons and dicotyledons. We show that resistance in barley requires a SNAP-25 (synaptosome-associated protein, molecular mass 25 kDa) homologue capable of forming a binary SNAP receptor ( SNARE) complex with ROR2. Genetic control of vesicle behaviour at penetration sites, and plasma membrane location of PEN1/ROR2, is consistent with a proposed involvement of SNARE-complex-mediated exocytosis and/or homotypic vesicle fusion events in resistance. Functions associated with SNARE-dependent penetration resistance are dispensable for immunity mediated by race-specific resistance (R) genes, highlighting fundamental differences between these two resistance forms.
引用
收藏
页码:973 / 977
页数:5
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