An efflux pump is involved in secretion of newly synthesized siderophore by Pseudomonas aeruginosa

被引：57

作者：

Hannauer, Melissa ^{[2
]}

Yeterian, Emilie ^{[2
]}

Martin, Lois W. ^{[1
]}

Lamont, Iain L. ^{[1
]}

Schalk, Isabelle J. ^{[2
]}

机构：

[1] Univ Otago, Dept Biochem, Dunedin 9054, New Zealand

[2] ESBS, FRE 3211, F-67413 Strasbourg, France

来源：

FEBS LETTERS | 2010年 / 584卷 / 23期

关键词：

Siderophore; Efflux pump; Metal trafficking; Iron transport; Secretion; Pseudomonas aeruginosa; FERRIPYOVERDINE RECEPTOR; PYOVERDINE BIOSYNTHESIS; SEQUENCE-ANALYSIS; ESCHERICHIA-COLI; OUTER-MEMBRANE; FPVA RECEPTOR; IRON; FLUORESCENT; IDENTIFICATION; ENTEROBACTIN;

D O I：

10.1016/j.febslet.2010.10.051

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pseudomonas aeruginosa secretes the fluorescent siderophore, pyoverdine (PVD), to enable iron acquisition. Epifluorescence microscopy and cellular fractionation were used to investigate the role of an efflux pump, PvdRT-OpmQ, in PVD secretion. Bacteria lacking this efflux pump accumulated PVD, or a fluorescent precursor, in the periplasm, due to their inability to efficiently secrete into the media newly synthesized PVD. PvdRT-OpmQ is only the second system identified for secretion of newly synthesized siderophores by Gram negative bacteria. (c) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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页码：4751 / 4755

页数：5

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